养阴益气活血冲剂对脑缺血-再灌注损伤保护作用的实验研究  

Experimental studies on protective effects of Yangyin Yiqi Huoxue infusion (养阴益气活血冲剂) on cerebral ischemia-reperfusion injury

作　　者：万海同[1] 白海波[2] 杨洁红 黄绳武[1] 王立人[1] 蔡宇[2] 严伟民[2] 郑筱祥[2] 

机构地区：[1]浙江中医学院,浙江杭州310053 [2]浙江大学生物医学工程学系,浙江杭州310027

出　　处：《中国中西医结合急救杂志》2001年第2期85-87,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目! (No.3 980 0 192 );国家自然科学基金资助项目! (No.3 0 0 70 941);浙江省自然科学基金青年科技人才培养

摘　　要：目的 :探讨养阴益气活血冲剂对脑缺血再灌注损伤的保护作用。方法 :制作 SD大鼠脑缺血与再灌注损伤模型 ,随机分为假手术对照组、脑缺血再灌注损伤组、脑缺血组、养阴益气活血冲剂不同剂量治疗组及维脑路通对照治疗组 ,观察各组脑组织前列环素 (PGI2 )和血栓素 A2 (TXA2 )含量 ;脑组织超氧化物歧化酶(SOD)活性和丙二醛 (MDA)含量 ;血浆组织型纤溶酶原激活物 (t PA)及其抑制物 (PAI)活性等指标的变化。结果 :养阴益气活血冲剂可显著提高脑组织 PGI2 含量 ,降低 TXA2 含量 ;调节血浆 t PA与 PAI的活性 ;显著降低脑组织 MDA含量 ,提高脑组织 SOD活性。结论 :养阴益气活血冲剂具有抗凝、提高纤溶活性、提高细胞抗氧化等多种作用 。Objective:To explore the protective effects of Yangyin Yiqi Huoxue infusion (YYYQHXI,养阴益气活血冲剂) on the cerebral ischemiareperfusion injury (CIRI).Methods:The rat models with CIRI were prepared and randomly divided into shamoperative group,CIRI group,ischemia group,treated group with different dosage of YYYQHXI,and venoruton control group.The changes in following parameters,including the contents of prostacyclin I 2 (PGI 2) and thromboxin A 2 (TXA 2) in brain tissue,the activites of superoxide dismutase (SOD) and the contents of malonyldialdehyde (MDA) in even pulp of brain and the activities of tissue plasminogen activator (tPA) and its inhibitor (PAI) were observed.Results:YYYQHXI was able to increase the contents of PGI 2 and decrease the contents of TXA 2 in brain tissue obviously,to regulate the activities of plasma tPA and PAI,to reduce the content of MDA obviously,and to heighten the activity of SOD in brain tissue.Conclusions:YYYQHXI possesses anticoagulative,increasing fibrolytic,promoting antioxidative,and additional effects so that it has significantly protective actions on CIRI.

关 键 词：滋阴 缺血-再灌注损伤 养阴益气活血冲剂 中医药疗法 

分 类 号：R285.5[医药卫生—中药学]