棉花枯萎病菌生理型Ⅱ、Ⅲ号原生质体形成和再生研究  

ON THE OCCURRENCE AND REPRODUCTION OF PHYSIOLOGICAL FORM Ⅱ AND Ⅲ OF FUSARIUM OXYSPORUM F.VASINFECTUM (ATK.) SNYDER ET HANSEN

作　　者：田逢秀[1] 史大刚[1] 宋天凤 徐孝华[2] 

机构地区：[1]新疆农科院植保研究所 [2]北京农业大学

出　　处：《植物病理学报》1989年第4期235-238,共4页Acta Phytopathologica Sinica

摘　　要：通过对棉花枯萎镰刀菌生理型Ⅱ、Ⅲ号原生质体的分离、纯化和再生条件的研究,摸清了同龄小型分生孢子在25℃下振荡培养30—40小时的菌丝,在0.6MNacl为稳定剂、4%的混合酶、39℃下酶解3—4小时能得到较高数量的原生质体。在高渗培养基上培养12小时即出现再生,2—3天后再生率达到66—83%。这一工作为进一步研究致病力变异奠定了基础。Studies on the reproduction of plastid from the physiological forms Ⅱand Ⅲ of Fusarium oxysporum f.vasinfectum (Atk.) Snyder et Hansen re-vealed that allarge number of plastid were obtained from the same aged hyphaewhich had been bred vibrationally at 25℃ for 30—40 hrs and then isolatedenzymatically at 39℃ for 3—4 hrs with 0.6M NaCl as stabilizer and 4% mixedenzyme.Plastid reproduction occurred in 12 hrs when it was cultured on thehigh-osmotic culture medium and the reproduction rate reached 66—83% after2—3 days.This has laid a foundation for further studies of variation of thepathogenicity.

关 键 词：棉花 枯萎病 镰刀菌 生理Ⅱ型 生理Ⅲ型 原生质体形成 原生质体再生 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治] S432.44[农业科学—植物保护]