用RAPD技术鉴定两个小冰麦易位系  

The Identification of Two Agropyron intermedium Translocation Lines in Wheat by RAPD Analysis

作　　者：鲍晓明[1] 黄百渠[1] 李松涛[2] 张忠廷[2] 王斌[2] 

机构地区：[1]东北师范大学遗传与细胞研究所,长春130024 [2]中国科学院遗传研究所

出　　处：《Acta Genetica Sinica》1993年第1期81-87,共7页

摘　　要：本文对从小冰麦异附加系中选育出的两个抗锈品系小冰麦33号,小冰麦34号及其原始亲本新曙光一号和抗源供体天兰冰草进行了RAPD分析,确定了小冰麦33号和小冰麦34号是含一段冰草DNA的易位系。文中还讨论了RAPD做为一种准确、快速鉴定易位系的方法的可行性。To identify two rust resistant Wheat-wheatgrass No.33 and No.34 derived from a whea'-grass aline addition line, we analyzed them and their parents (commom wheat Xinshuguang No. 1 and wheatgrass) by RAPD method. The results suggest that Wheat-wheatgrass No.33 and No.34 are both translocation lines and their resistant genes come from wheatgrass. Furthermore, RAPD is a simple, rapid and efficient method for the identification of translocation lines.

关 键 词：易位 RAPD 小冰麦 天兰冰草 抗锈生状 

分 类 号：S512.103.4[农业科学—作物学]