^（125）I－睾酮固相分离放射免疫分析法的应用评估  

EVALUATION OF THE APPLICATION FOR ^(125)I-TESTOSTERONE RADIOIMMUNOASSAY BY SOLID PHASE SEPARATION

作　　者：汤涛[1] 

机构地区：[1]安徽省立医院内分泌实验室

出　　处：《同位素》1995年第4期244-246,共3页Journal of Isotopes

摘　　要：￣（１２５）Ｉ－睾酮固相分离放射免疫分析法的观察结果表明，该法灵敏度、准确度高，重复性好。灵敏度为０．１５ｎｍｏｌ／ｌ；批内ＣＶ＝５．７％，批间ＣＶ＝１５．１％，免疫反应时间为２－３ｈ。反应１２０、１５０和１８０ｍｉｎ的结果（ｘ±ＳＤ）分别为５４．４３±３．５１％，５０，６３±４．３５％和５３．２３±２．２１％，Ｂ／Ｔ结果基本稳定。与液体闪烁技术同时测定性腺疾病门诊男、女病人，其结果与各自相应正常参考值比较，两者符合率为９４．１％。?125)I-serum testcoterone radioimmunoassay by the solid phase separation is evaluated. Themethod's sensitivity is 0.15 mol/l.The intro- assay CV is about 5.7%and the inter- assay CV isabout 15.1%. The immune reaction is steady basically in 2-3h.The B/T value(x±SD)is 54.43%±3.51%,50.63%±4.35%and 53.23%±2.21%respectively for the reaction time of120,15 0 and 1 8 0 min. The RIA determination of serum or plasma testorterene for male's and female's sex disease out-patients is performed with both solid phase separation and liquid scintillationtechnique at the same time. Comparing with each relevent normal range,the results indicate that thecoincident rate is 94.1%.

关 键 词：睾酮 固相分离 放射免疫分析 碘125 

分 类 号：R817.4[医药卫生—影像医学与核医学] R446.6[医药卫生—放射医学]