应用酵母双杂交研究G蛋白通路中的蛋白因子  

Hunting for Novel Protein Factors in G-protein Pathway with Yeast Two-hybrid System

作　　者：鲁宁[1] 李旌军[1] 黄秉仁[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所生化室,北京100005

出　　处：《中国医学科学院学报》2001年第2期111-114,I003,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金!( 39630120)&&

摘　　要：目的 探寻 G蛋白信号传导途径中与 G_β亚基相互作用的下游蛋白因子，揭示 G蛋白信号传导通路的机制。 方法 采用敏感性较高的酵母双杂交体系，构建含 G_β亚基基因的饵质粒筛选人脑 cDNA文库。结果 获得了肌动蛋白集束调控蛋白 (actin bundling protein)的编码基因和两个新基因片段， GeneBank登录号分别为 AF288405（ 427 bp）及 AF288406（ 2 832 bp）。结论 提示在人脑组织中 G_β亚基作为结构和功能单位可能通过与 actin bundling protein及另两种未知的蛋白因子间的相互作用而介导了信号的传导，对于揭示 G蛋白信号传递通路与 actin细胞骨架之间的关系起重要的提示作用。Objective To explore the protein factors that could interact with G_β subunit within the G protein signal transducing pathway. Methods The highly sensitive protein-protein interaction system── Yeast Two-hybrid System was applied to screen the human cDNA library with constructed“ Bite plasmid” containing G_β subunit gene fragment. And then the faulse positive test was adapted. Results Three positive gene fragments were obtained. One codes for“ Actin bundling protein” . The other two are new ones and their GeneBank accession numbers are AF288405 and AF288406 respectively. Conclusions It is the first time to find that among human brain tissue, G_β subunit becomes a structural or functional unit interacts with actin bundling protein and the other two unknown protein factors to activate the following pathway. This result may be important to understand the relationship between the actin cytoskeleton and G proteins.

关 键 词：G蛋白 Gβ亚基 蛋白-蛋白相互作用 酵母双杂交系统 

分 类 号：Q503[生物学—生物化学]