趋化素样因子(CKLF1)对骨髓细胞增殖活性的研究  

Stimulatory Effect of Chemokine-like Factor 1 (CKLF1 ) on theGrowth of Bone Marrow Cells

作　　者：韩文玲[1,2] 芮珉[1] 张颖妹[1] 陈英玉[1] 钟英成[1] 狄春辉[1] 宋泉声[1] 马大龙[1] 

机构地区：[1]北京大学基础医学院免疫系 [2]Fax: [phone]

出　　处：《中国医学科学院学报》2001年第2期119-122,共4页Acta Academiae Medicinae Sinicae

基　　金：国家高技术研究发展计划 863课题!( 102-08-07-04-99)&&

摘　　要：目的 研究趋化素样因子 1(CKLF1)对造血功能的调节作用。方法 利用 MTT法，检测 CKLF1真核细胞表达载体转染上清在液体培养基中，对人低密度骨髓细胞和小鼠骨髓细胞的增殖调控作用；并在半固体培养基中，观察其对人低密度骨髓细胞集落形成的刺激作用。结果 COS-7细胞表达的 CKLF1能明显促进人和小鼠骨髓细胞的增殖，并能促进人骨髓细胞的集落形成，与 GM-CSF有协同刺激作用。结论 CKLF1能促进人和小鼠骨髓造血干Objective To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. Methods Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added.The cell proliferation was assayed by MTT method after 5 days incubation.The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture. Results CKLF1 has obvious enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. Conclusion CKLF1 can promote the proliferation and differentiation of bone marrow cells.

关 键 词：趋化素样因子1 造血干细胞 集落形成 骨髓细胞增殖 

分 类 号：R392.11[医药卫生—免疫学] R331.22[医药卫生—基础医学]