合成鱼腥草素对脾切除动物细胞免疫功能的影响  

The effect of Houttuyninum on cellar immunoiogic function in splenectomy animals

作　　者：邵兰[1] 于庆海[2] 黄晴[1] 刘树森[1] 

机构地区：[1]中国科学院动物研究所生物膜与膜生物工程国家重点实验室,北京100080 [2]沈阳药科大学药理教研室,沈阳110015

出　　处：《中国药理学通报》2001年第1期51-54,共4页Chinese Pharmacological Bulletin

摘　　要：目的　研究合成鱼腥草素对脾切除动物细胞免疫功能的影响及作用机制。方法　建立小鼠、大鼠脾切除模型 ,测定迟发型超敏反应强度、外周血淋巴细胞ANAE阳性百分率、胸腺T细胞增殖能力、淋巴结T细胞增殖能力、淋巴结中淋巴细胞数量、外周血T细胞亚群。结果　合成鱼腥草素ig (6 0mg·kg-1,12 0mg·kg-1)能明显增强脾切除小鼠迟发型超敏反应强度 ,ConA诱导的淋巴结T淋巴细胞增殖能力 ,增加外周血淋巴细胞ANAE阳性百分率、淋巴结中淋巴细胞数量 ,而对胸腺T淋巴细胞增殖能力则无明显影响 ;合成鱼腥草素ig (4 0mg·kg-1,80mg·kg-1)明显升高脾切除大鼠Th 数量 ,降低Ts 数量 ,调节外周血T淋巴细胞亚群Th/Ts 比值。结论　合成鱼腥草素对脾切除动物细胞免疫功能具有调节作用 。AIM To investigated the effect and mechanism of Houttuynium (HOU) on splenectomy animals. METHODS The models of splenectomy mice and splenectomy rats were made and delayed type hypersensitivity (DTH) reaction, ANAE (+) cell percentage of peripheral blood lymphocytes, Con A induced thymus and groin lymph node lymphocyte proliferation, the amount of lymphocytes of groin lymph node,and T cell subpopulation in peripheral blood were measured. RESULTS HOU ig (60 mg·kg -1 , 120 mg·kg -1 ) can strikingly enhanced delayed type hypersensitivity (DTH) reaction, ANAE (+) cell percentage of peripheral blood lymphocytes. strengthened Con A induced groin lymph node lymphocyte proliferation, raised the amount of lymphocytes of groin lymph node in splenectomy mice. But the effects of them on Con A induced thymus lymphocyte proliferation were not apparent. HOU ig (40 mg·kg -1 , 80 mg·kg -1 ) improved T h subset, reduced T s subset, and then modulated the rate of T h/T s in splenectomy rats. CONCLUSION HOU strengthened the cellar immune function of splenectomy animals by promoting the compensating capability of lymph node and regulating T cell subpopulation.

关 键 词：合成鱼腥草素 脾切除 细胞免疫 T细胞增殖 免疫功能 实验研究 

分 类 号：R285.5[医药卫生—中药学] R657.6[医药卫生—中医学]