成骨细胞跨膜转运锌特性及其机制的初步研究  

Primary study of transmembrane transport of zinc and its mechanism in osteoblast

作　　者：岑小波[1] 王瑞淑[2] 王莉[1] 王航[3] 

机构地区：[1]华西医科大学附属第一医院国家中药安全性评价中心,成都610041 [2]华西医科大学公共卫生学院,成都610041 [3]华西医科大学口腔医学院,成都610041

出　　处：《中国药理学通报》2001年第1期83-86,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目!No 39770 6 47

摘　　要：目的　研究锌在成骨细胞跨膜转运的动力学特点及影响因素 ,为阐明锌在骨发育中的作用提供科学依据。方法　采用65Zn同位素示踪法研究锌在成骨细胞跨膜转运的动力学特点 ;分别研究组氨酸、Na+、K+泵抑制剂、Ca2 +离子通道阻滞剂对成骨细胞锌转运的影响。结果　细胞外锌浓度增加可以促进锌转运入胞内 ,但锌缺乏使锌胞内转运减少 ;组氨酸、Na+、K+泵抑制剂对锌转运无影响 ;Ca2 +离子通道阻滞剂促进锌内流。结论　成骨细胞外环境锌水平可以影响成骨细胞锌转运及胞内锌水平 ,Ca2 +离子通道与Zn2AIM To study the kinetic character of zinc transport and its influencing factors in osteoblasts, and scientific evidence is expected to provide to clarify the role of zinc on bone development. METHODS 65 Zn tracing method was used to evaluate kinetic character of zinc transport. RESULTS Increase of extracellular zinc level could enhance the influx of zinc, but when the osteoblast became zinc deficiency the influx of zinc decreased. The histidine, Na +、K + ATPase enzyme inhibitor had no effect on zinc transport into the cell, but Ca 2+ channel blocker could enhance the influx of zinc. CONCLUSION The excellular level of zinc could influence zinc transport into osteoblasts, and Ca 2+ channel could interact with Zn 2+ transport.

关 键 词：锌 成骨细胞 细胞培养 锌转运 体外培养 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R322.71[医药卫生—基础医学]