一氧化碳合成酶抑制剂对缺氧性肺血管收缩反应的影响  

Effect of carbon monoxide synthase inhibitor on hypoxic pulmonary vasoconstriction

作　　者：丁学琴[1] 马虹[1] 盛卓人[1] 刘贵明[1] 王俊科[1] 

机构地区：[1]中国医科大学第一临床学院麻醉科,沈阳市110001

出　　处：《中华麻醉学杂志》2001年第4期226-228,共3页Chinese Journal of Anesthesiology

摘　　要：目的　观察内皮及内源性一氧化碳 (CO)合成酶抑制剂 (血红素氧化酶抑制剂ZnppIX)对大鼠缺氧性肺血管收缩反应的影响 ,探讨内源性CO在缺氧性肺血管收缩反应中的作用。方法　制备Wistar大鼠肺动脉环 ,观察内皮与缺氧性肺血管收缩反应的关系 ;并以一氧化氮合成酶抑制剂L NAME为对照 ,观察ZnppIX对缺氧性肺血管收缩反应的影响。 结果　缺氧可使去氧肾上腺素预收缩的肺动脉环出现明显的收缩反应 ,去除内皮或血管环用L NAME孵育后 ,缺氧性肺血管收缩反应受抑 ,而用ZnppIX及L NAME共同孵育后 ,缺氧性肺血管收缩反应明显抑制或消除 ,与L NAME组相比有显著性差异 (P <0 0 1)。L NAME组的缺氧张力变化率与未孵育前相比也有显著性差异(P <0 0 1)。结论　缺氧性肺血管收缩反应是内皮依赖性的 ,ZnppIX可抑制大鼠缺氧性肺血管收缩反应 ,内源性CO与NO一样参与了缺氧性血管收缩反应。Objective To investigate the effect of heme oxygenase inhibitor ZnppIX, an endogenous carbon monoxide(CO) synthase inhibitor,on hypoxic pulmonary vasoconstriction(HPV)of isolated rat pulmonary arterial ring(PAR), trying to explore the role of endothelium and endogenous CO in HPV Methods Male Wistar rats (260 350g) were used PAR (4 mm long) was prepared immediately after the animal was decapitated and suspended in Krebs Hansleit(KH) solution maintained at 37℃ aerated with 5% CO 2 in O 2 The arterial ring connected to a transducer polygraph assembly for the measurement of the tension of PAR Firstly the integrity of the endothelium of the arterial ring was tested and confirmed In one group PAR was denuded of enothelium, then HPV of PAR was tested PAR was preconstricted by phenylepinephrine(PE) 1μmol/L and tension was recorded as P PE When tension reached a plateau, the KH solution was aerated with 95%N 2 5%CO 2 and maintained for 30 min The tension induced by hypoxia,was recorded as P H The change in tension was calculated as P% After HPV test the KH solution was washed for 30 min and the study was carried out then in two groups: L NANE group (control group) and ZnppIX+L NANE group Nitric oxide synthase inhibitor, L NANE(100 μmol/L) was added and incubated for 30 min and then HPV test was performed in control group In ZnppIX+L NANE group, ZnppIX(10 μmol/L)and L NANE(100 μmol/L) were added and after 30min incubation the HPV test was conducted The P PE ,P H and P% before and after incubation and between the two groups were compared Results Hypoxia caused vasoconstriction of PAR preconstricted by PE Removal of endothelium or L NANE pretreatment decreased the magnitude of constriction induced by hypoxia However pretreatment with ZnppIX and L NANE markedly decreased or abolished the HPV as compared with control group(P<0 01) Conclusions HPV is endothelium dependent ZnppIX can inhibit HPV CO as well as NO plays an important role in HPV

关 键 词：一氧化碳 一氧化碳 血红素氧化酶抑制剂 缺氧性肺血管收缩 

分 类 号：R614[医药卫生—麻醉学]