蝙蝠葛苏林碱对家兔在体心脏单相动作电位的影响(英文)  

Effect of daurisoline on monophasic action potential of rabbit hearts in vivo

作　　者：李真[1] 林先明[2] 夏敬生[1] 杨晓燕[1] 龚培力[1] 曾繁典[1] 

机构地区：[1]华中科技大学同济医学院临床药理学研究所,湖北武汉430030 [2]华中科技大学同济医学院药理学教研室,湖北武汉430030

出　　处：《中国药理学与毒理学杂志》2001年第2期116-120,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目! ( 396 70 834 )&&

摘　　要：采用单相动作电位记录技术研究蝙蝠葛苏林碱 (DS)对家兔在体心脏单相动作电位 (MAP)作用的使用依赖性特征 .结果显示DS在 2 4 0 ,2 10 ,180ms起搏周长 (CL)均能降低MAP振幅 (MAPA) ,延长MAP复极达 50 %和 90 %的时程 (MAPD50 ,MAPD90 ) ,有效不应期 (ERP)和ERP/MAPD90 .延长MAPD50 百分率分别为 12 .8± 2 .9,9.5± 2 .6 ,9.0± 1.6 ,延长MAPD90 百分率分别为 10 .9± 2 .6 ,7.5± 2 .4 ,6 .5±2 .7,延长ERP百分率分别为 2 5.7± 4 .3,18.5±4 .1,2 4 .2± 7.2 ,延长ERP/MAPD90 百分率分别为13.7± 4 .5,10 .2± 2 .2 ,16 .1± 5.1(P >0 .0 5) .其延长作用均呈非使用依赖性特征 .在所有起搏周长下 ,索他洛尔亦降低MAPA ,延长MAPD50 ,MAPD90 ,ERP ,ERP/MAPD90 ,但其延长MAPD90 ,ERP的作用均呈逆向使用依赖性特征 (P <0 .0 5) .结果表明 ,DS能明显降低家兔在体心脏MAPA ,延长MAPD50 ,MAPD90 ,ERP ,ERP/MAPD90 ,该作用呈非使用依赖性特征 .The use-dependent characteristics of daurisoline on monophasic action potentials (MAP) of the left ventricle of the rabbit heart in situ were recorded with MAP recording technique. The results showed that daurisoline decreased monophasic action potential amplitude (MAPA) and prolonged monophasic action potential duration at the 50% and 90% (MAPD 50 , MAPD 90 ), effective refractory period (ERP), ERP/MAPD 90 at the steady-state cycle lengths of 240, 210, 180 ms. The percent of prolongation of MAPD 50 was 12.8±2.9, 9.5±2.6, 9.0±1.6, that of MAPD 90 was 10.9±2.6, 7.5±2.4, 6.5±2.7, that of ERP was 25.7±4.3, 18.5±4.1, 24.2±7.2, and that of ERP/MAPD 90 was 13.7±4.5, 10.2±2.2, 16.1±5.1, respectively. Its effect showed no relation to frequency (n=6, P>0.05). The effects of sotalol on MAP were similar to that of daurisoline, but it prolonged MAPD 90 and ERP in a reverse use-dependent manner (P<0.05). It is concluded that daurisoline has no use-dependent effects on MAP of rabbit hearts.

关 键 词：蝙蝠葛苏林碱 心脏 电位测定法 动作电位 使用依赖性 

分 类 号：R965[医药卫生—药理学]