预适应和前胡丙素对缺氧肥厚血管平滑肌胞内钙离子浓度及NO含量的影响  

Effects of preconditioning and praeruptorine C on intracellular free calcium level and NO content in hypertrophied vascular smooth muscle cells with hypoxia

作　　者：饶曼人[1] 刘宛斌[1] 张晓文[1] 

机构地区：[1]南京医科大学心血管药理研究室,江苏南京210029

出　　处：《中国药理学与毒理学杂志》2001年第2期141-144,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目! ( 395 70 819)&&

摘　　要：观察了预适应和前胡丙素对缺氧肥厚血管平滑肌胞内钙离子浓度 ([Ca2 +]i)及NO含量的影响 .①建立两肾一夹肾血管性高血压大鼠模型 ,分离培养主动脉血管平滑肌细胞 (VSMC) ,以fura 2 /AM为钙指示剂。测得前胡丙素 (2 0mg·kg- 1·d- 1,术后第9周起ig 9周 )治疗和缺氧预适应 (5minN2 ,5min95%O2 + 5%CO2 混合气体 ,循环 3次 )对缺氧 (30minN2 )所致肥厚VSMC对KCl和去甲肾上腺素刺激反应性升高 ([Ca2 +]i 升高 )有明显的拮抗效应 .②用血管紧张素Ⅱ刺激致VSMC肥厚 ,前胡丙素 (10μmol·L- 1温育 2 4h)和缺氧预适应合用使缺氧肥厚VSMC的NO含量恢复至正常VSMC水平 .结果提示前胡丙素与缺氧预适应对肥厚VSMC缺氧损伤有协同性的保护作用 .Effects of preconditioning(PC) and praeruptorine C(Pra-C) on intracellular free calcium level([Ca 2+ ] i) and NO content in hypertrophied vascular smooth muscle cells (VSMC) with hypoxia were studied. ①The aorta VSMC of rats with renovascular hypertension induced by two-kidney-one-clip(2K1C) were isolated and cultured. Fura-2/AM was as a calcium fluorescent indicator. It was seen that Pra-C(20 mg·kg -1 ·d -1 ,ig, for 9 weeks from week 9 after 2K1C) and PC (3 cycles of 5 min pure N 2 and 5 min 95%O 2+5%CO 2) antagonisted the elevated activities(higher [Ca 2+ ] i) induced by KCl and norepinephrine in VSMC with hypo- xia (30 min pure N 2). ② In hypertrophied VSMC stimulated by angiotensinⅡ, combination of Pra-C(10 μmol·L -1 for 24 h) and PC enhanced NO content to the normal level in hypertrophied VSMC with hypo- xia. The results suggest that combination of Pra-C and PC have cooperative protection for hypertrophied VSMC with hypoxia injury.

关 键 词：前胡丙素 缺血预适应 心肌缺血 一氧化氮 血管平滑肌细胞 钙离子浓度 

分 类 号：R965[医药卫生—药理学]