骨髓CFU-F体内、体外成骨功能研究  

Study on bone marrow CFU-F and soteogenesis in vivo and in vitro

作　　者：刘雅娟[1] 王莉馨[1] 林军 于洪臣[1] 范洪学[1] 

机构地区：[1]吉林大学预防医学院,吉林长春130021 [2]北京中国饲料集团公司

出　　处：《吉林医学》2001年第2期69-70,共2页Jilin Medical Journal

摘　　要：目的：研究骨髓基质成纤维细胞与成骨的关系，为临床应用奠定基础。方法：采用Ｌｉｕｒｉａ多器官培养器体外器官培养法、肾被膜下移植技术以及石蜡切片技术、Ｇｏｍｏｒｉ染色和Ｖｏｎ Ｋｏｓｓａ染色技术。结果：移植物在肾被膜下形成软骨细胞团及骨基质；在β－ＧＰ存在下，骨髓基质ＣＦＵ－Ｆ体外培养可见形成钙化的骨板。结论：骨髓基质ＣＦＵ－Ｆ为基质多能干细胞，能增生分化为成骨以及造血诱导微环境所必需的各种基质细胞系。Objective To study the reationship between bone marrow stromal cells and osteogenesis for clinical application. Method The Luria organ culture in vitro, transplantation under the renal capsule and paraffin sections, Gomori-dye and Von Kossa-dye were used. Results Cartilage and bone stroma were foumed under the renal capsule and calcificated bone were observed in vitro in the presence of β- GP.Conclusion Bone marrow CFU-F are pluripotential stromal stem cells which are capable of proliferating and differentiating into all stromal cells lines necessary for the formation of bone and reconstitution of the hematopoietic inductive microenvironment.

关 键 词：骨髓 基质成纤维细胞 体内成骨 体外成骨 

分 类 号：R68[医药卫生—骨科学]