四逆汤调节大鼠缺血心肌β受体信号转导的机制  

Mechanism of Sini Decoction in Regulating β-Adrenergic Receptor Signal Transduction in Rats with Myocardial Ischemia

作　　者：唐铁军[1] 吴伟康[1] 卢汉平[2] 刘秀琴[2] 别平华[3] 

机构地区：[1]中山医科大学中西医结合研究所,广州510089 [2]中山医科大学放射免疫检测中心 [3]第一军医大学微生物学教研室

出　　处：《中国中西医结合杂志》2001年第3期206-208,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：中国博士后基金资助项目!〔1 999〕1 0号;广东省中医药管理局基金资助项目!(No.99564)

摘　　要：目的 :研究四逆汤对大鼠心肌缺血 β肾上腺素能受体 ( β AR)信号转导的影响。 方法 :用心得安阻断β AR ,再以垂体后叶素造成大鼠心肌缺血 ,采用放射配基受体结合分析法测定心肌细胞膜β AR密度 ,放射免疫法测定大鼠血浆及心肌组织中cAMP水平 ,定量RT PCR法测定β1 AR及 β1 AR激酶 ( βARK 1)mRNA的表达。结果 :心肌缺血时β AR密度上调 ,而血浆及心肌cAMP水平却低于正常组 (P <0 0 1和P <0 0 5) ,βARK 1mRNA表达增加 (P <0 0 1) ;四逆汤能促进β1 ARmRNA表达 ,抑制 βARK 1mRNA表达 ,从而增加 β AR密度 ,使血浆和心肌cAMP水平明显上升。 结论 :四逆汤能减少心肌缺血时的 β1 AR脱敏 ,促进心肌β AR信号转导。Objective: To investigate the effect of Sini Decoction (SND) on β adrenergic receptor (β AR) signal transduction in rats with myocardial ischemia. Methods: After β AR had been blocked by propranolol, the myocardial ischemia rats were established by pituitrin injection. The density of β AR in myocardial cell membrane was determined by radioligand binding assay, cAMP level in plasma and myocardial tissue was detected by radioimmunoassay (RIA), and the mRNA expression of β 1 AR and β 1 AR kinase (βARK 1) was measured by quantitative reverse transcriptase polymerase chain reaction (RT PCR) amplifying assay. Results: In myocardial ischemia group, the density of β 1 AR was up regulated and the mRNA expression of βARK 1 increased (P<0 01), but the cAMP level in plasma and myocardium was lower than that in the normal group (P<0 01 and P<0 05). SND could promote β 1 AR mRNA expression and inhibit βARK 1 mRNA expression so as to increase the density of β AR and cause significant raising of cAMP level in plasma and myocardium. Conclusion: SND could reduce the desensitization of β 1 AR during myocardial ischemia and improve signal transduction of β AR.

关 键 词：四逆汤 心肌缺血 Β肾上腺素能受体 信号转导 脱敏 

分 类 号：R285.5[医药卫生—中药学]