骨性关节病与磷脂酶A_2关系的研究  

An Investigation Of Phosphlipase A_2 Actvity In Synovial Fluids In Osteoarthritis

作　　者：宣靖[1] 李世和[1] 王宏邦[1] 曾才铭[1] 

机构地区：[1]昆明医学院附一院骨科,650031

出　　处：《骨与关节损伤杂志》2001年第2期122-124,共3页The Journal of Bone and Joint Injury

摘　　要：目的　探讨磷脂酶A2 (APLA2 ) ,前列腺素E2 (PGE2 ) ,PH在骨性关节病 (OA)中的作用及关节液中PLA2 与PGE2 的关系。方法　抽取 2 8例OA患者 ,14例对照者膝关节液 ,采用酶联免疫法测定PGE2 ,生物活性法测定PLA2 ,用 5 80 0 - 0 5溶液分析仪测定PH值。结果　OA组PLA2 活性高于对照组 (P <0 0 0 1) ;OA组PGE2 含量高于对照组 (P <0 0 0 1) ;OA组PH值低于对照组 (P <0 0 5 ) ;PLA2 活性与疼痛程度 ,病情评分呈正相关 (P <0 0 0 1) ;PLA2 活性与PGE2 含量呈正相关 (P <0 0 0 1)。Objective To study the relationship between PLA 2,PGE 2,PH and osteoarthritis, at the same time to elucidate the relationship between PLA 2 and PGE 2 Methods PLA 2, PGE 2 PH were measured concurrently in synovial fluid samples from 28 patients with osteoarthritis ( OA) and from 14 normal The determination of PGE 2 was carried out using ELISA The determination of PLA 2 was performed by bioassay The PH was get by using 5800-05 Solution Analyzer Results The activities of PLA 2 are higher in synovial fluids from OA than those from normal (P<0 001) The concentrations of PGE 2 ane higher in synovial fluids from OA than those from normal (P<0 05) The PH are lower in synovial fluids from OA than those from normal (P<0 05) In the OA synovial fluids, the level of PLA 2 activity is correlated to the severity of pain The level of PLA 2 actvity is correlated to the concentration of PGE 2 Conclusion The level of PLA 2 activity and the concentration of PGE 2 play an important role in the pathogenesis of OA The higher level of PLA 2 activity may be the reason of symptom aggravating The PLA 2 activity affects the concentration of PGE 2

关 键 词：骨性关节病 膝关节 疼痛 磷脂酶A2 前列腺素E2 

分 类 号：R684[医药卫生—骨科学]