Benazepril和Losartan对培养SHR心肌成纤维细胞增殖及合成胶原影响  

Effects of Benazepril and Losartan on Proliferation and Collagen \;Synthesis of Cultured Cardiac Fibroblasts from SHR

作　　者：谢良地[1] 陈水龙[1] 许昌声[1] 王华军[1] 吴可贵[1] 

机构地区：[1]福建省高血压研究所

出　　处：《高血压杂志》2001年第1期62-64,共3页Chinese Journal of Hypertension

基　　金：福建省自然科学基金!(NO :C970 44 );后备人选培养基金!(99J12 3)资助课题

摘　　要：目的　观察血管紧张素Ⅱ (AngⅡ )、苯那普利 (Benazepril,Be na)、洛沙坦 (Losartan)对培养的SHR和WKY大鼠心肌成纤维细胞 (CFb)增殖及合成胶原的影响。方法　采用组织块贴壁法培养CFb ,分别测定AngⅡ、Bena干预下CFb的增殖情况 ,AngⅡ、Bena和Losartan干预下CFb的3H 脯氨酸 (3H Proline)掺入量。结果　各种浓度的AngⅡ (10 -10 mol～ 10 -6mol)和Bena(10 -9mol～ 10 -5mol)对CFb的细胞数显著增殖无影响 ,AngⅡ呈浓度依赖性促进CFb的3H Proline掺入量 ,Bena抑制CFb的3H Proline掺入量 ,Losartan呈浓度依赖性抑制 10 -7molAngⅡ诱导的CFb的胶原合成。结论　AngⅡ和Bena不影响CFb的增殖 ,AngⅡ对CFb的胶原合成有促进作用 ,Bena抑制CFb胶原合成 。Objective\ To investigate the effects of angiotensinⅡ(AngⅡ), Benazepril(Bena) and Losartan on proliferation and collagen synthesis of cultured rat cardiac fibroblasts(CFb) derived from SHR (CFb\-\{SHR\}) and WKY (CFb\-\{WKY\}). Methods\ CFb derived from 12\|week\|old SHR and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was determined by direct cell counting .\{\}\+3H\|Proline incorporation of CFb was measured after incubation with AngⅡ,Bena and Losartan. Results\ In serum free(0 4%FCS) medium, cell number of CFb derived from SHR and WKY were not influenced by AngⅡ(10\+\{-10\}mol～10\+\{-6\}mol)and Bena(10\+\{-9\}mol～10\+\{-5\}mol). Increased \{\}\+3H\|proline incorporation was induced by AngⅡ in a concentration dependent manner. Benazepril caused an decrease in \{\}\+3H\|Proline incorporation in CFb derived from SHR and WKY. The increased collagen synthesis induced by AngⅡ(10\+\{-7\}mol) was inhibited by Losartan(10\+\{-10\}mol～10\+\{-6\}mol) in a concentration dependent manner. Conclusion\ Proliferation of CFb were not influenced by AngⅡ and Bena. Collagen synthesis of CFb was promoted by AngⅡ and inhibited by Bena. Collagen synthesis of CFb induced by AngⅡ was inhibited by losartan.

关 键 词：心肌成纤维细胞 高血压 血管紧张素Ⅱ 苯那普利 洛沙坦 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]