中药软肝缩脾丸对肝纤维化大鼠TIMP-1/2蛋白表达的影响  

Effect of Rangansuopiwan in expression of tissue inhibitor of metalloproteinase-1/2 in rat liver fibrosis

作　　者：王全楚[1] 申德林[1] 张成道[1] 许丽芝[1] 聂青和[2] 谢玉梅[2] 周永兴[2] 

机构地区：[1]中国人民解放军153中心医院感染病科,河南省郑州市450042 [2]中国人民解放军第四军医大学唐都医院感染病科,陕西省西安市710038

出　　处：《世界华人消化杂志》2001年第4期379-382,共4页World Chinese Journal of Digestology

基　　金：河南省科委科研基金.No.96031~~

摘　　要：目的探索肝纤维化发生的分子机制,尤其是TIMP-1,TIMP-2的作用。方法采用免疫组化和原位杂交的方法分别测定5组肝纤维化大鼠不同治疗前后TIMP-1,TIMP-2的基因调节和蛋白表达。结果 CCl_4模型组TIMP-1,TIMP-2 mRNA及蛋白水平明显高于治疗组。正常组大鼠肝组织无一例阳性。结论 TIMP-1,TIMP-2与肝纤维化形成密切相关,软肝缩脾丸对TIMP-1,TIMP-2的基因和蛋白表达有一定的抑制作用。AIM To investigate the molecules responsible for the invasion potential of LC by focusing on tissue inhibitor of metalloproteinase (TIMP-2 and TIMP-1), because these enzymes participate in the degradation of the extracellular matrix including the basement membrane. METHODS Normal and hepatic fibrosis liver samples were obtained from CCI_4 damaged rats underwent Rangansuopiwan treatment. Transcripts for TIMP-1 and TIMP-2 or immunohistochemical examinations were detected in the liver tissues. RESULTS In 9 of the 12 LC samples, transcripts for TIMP-1 were detected in the liver tissues, and 5 of 12 of these samples showed stronger expression in the cirrhotic tissues than in the normal tissues. On the other hand, TIMP-2 messenger RNA (mRNA) was detected in 9 of the 12 cases. The expression of TIMP-1 mRNA in CCI_4 models was significantly higher than in treatment group. It tended to have a higher ratio of TIMP-1 mRNA expression to TIMP-2 mRNA expression. Immunohistochemical examinations revealed that TIMP-1 immunoreactivity was the most intense in the CCI_4 models. CONCLUSION TIMP-1 and TIMP-2 are closely related to hepatic fibrosis in LC. Rangansuopiwan can downregulate the expression of TIMP-1 and TIMP-2.

关 键 词：肝硬化 药物疗法 病理生理学 金属蛋白酶1组织抑制 中医药疗法 软肝缩脾丸 

分 类 号：R285.5[医药卫生—中药学]