高效液相色谱法测定羟基喜树碱及喜树碱含量  

The Determination of Hydroxycamptothecinum and Camptothecinum by HPLC

作　　者：马锦星 孙仲葆[1] 彭才勤[2] 马烈[2] 

机构地区：[1]湖北省黄石市药品检验所,黄石市435000 [2]武警湖北总队医院,武汉市430000

出　　处：《中国药房》2001年第3期172-173,共2页China Pharmacy

摘　　要：目的 :建立HPLC测定羟基喜树碱及喜树碱含量的方法。方法 :样品在弱碱性条件下溶解稀释成20μg/ml的溶液。色谱柱为YWGC18 柱 ,4 6mm×250mm ,10μm ;流动相为甲醇 -水 (45∶55) ;流速为1 0ml/min ;检测波长为266nm。结果 :羟基喜树碱回收率为100 2 % ,日间变异 (2、4、8h)RSD低于1 8 % ;喜树碱回收率为99 7 % ,日间变异 (2、4、8h)RSD低于2 0 %。结论 :本法分离效果好 ,专属性强 ,分析速度快 ,灵敏度高 。OBJECTIVE:To establish a HPLC assay for the determination of hydroxycamptothecinum and camptothecinum.METHODS:The concentrations of hydroxycamptothecinum and camptothecinum were assayed on a C18 column with a mobile phase consisting of methanol-water(45∶55) at a flow rate of 1.0 ml/min,and detected at 266nm.RESULTS:The mean recovery of hydroxycamptothecinum was 100.2%,the 2,4,8 hours precision RSD were less than 1.8%;The mean recovery of camptothecinum was 99.7%,the 2,4,8 hours precision RSD were less than 2.0%.CONCLUSION:The HPLC method for determination of hydroxycamptothecinum and camptothecinum was rapid,sensitive and accurate.

关 键 词：HPLC 羟基喜树碱 喜树碱 抗肿瘤药物 

分 类 号：R979.1[医药卫生—药品]