以浸蜡石墨电极为基体的过氧化物酶生物传感器  

An Amperometric Hydrogen Peroxide Sensor On Paraffin Soaked Graphite Electrode

作　　者：张国荣[1] 

机构地区：[1]山东师范大学化学系,济南250014

出　　处：《化学传感器》2001年第1期52-58,共7页Chemical Sensors

摘　　要：本文以浸蜡石墨电极为基体电极,将β—环糊精与环氧氯丙烷的交联聚合物(β—CDP)与亚甲蓝的超分子包合物(CMIC)和酶固定在电极上,制成了辣根过氧化物酶生物传感器。由于超分子包合物的形成将原本极易溶于水的电子转移介体——亚甲蓝固定在电极上,有效的减少了其流失,提高了该生物传感器的稳定性和寿命。该生物传感器对过氧化氢有电催化还原作用,电流法测定过氧化氢的线性范围为5.0×10^(-4)—1.0×10^(-2)mol·L^(-1),检出限为2.0×10^(-5)mol·L^(-1),达95%稳态响应电流所用时间小于40s。The polymer of β-cyclodextrin(β-CD) and epichlorohydrin has be synthesized in a strong base solution.Methylene blue (MB),The electron transfer mediator,and horseradish peroxidase (HRP) were co—immobilized on the electrode to fabricate the hy- drogen peroxide sensor by supramolecular function between β—CD polymer (β—CDP) and MB,as well as poly—condensation between β—CDP and glutaric dialdehye(GD).The for- mation of supramolecular greatly shortened the running—off of water—soluble mediator. The fabricated H_2O_2 sensor exhibited excellent stability and lifetime.The current recorded by amperometry with concentration of H_2O_2 in the range 5.0×10^(-5)—1.0×10(-2)mol/L with the detection limit of 2.0×10^(-5)mol·L^(-1).The response time was less than 40s.

关 键 词：浸蜡石墨电极 Β-环糊精聚合物 亚甲蓝 超分子作用 辣根过氧化物酶传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]