红豆杉细胞两液相体系中紫杉醇的分离研究  

Basic Study on Taxol Separation from the Organic Solvents in Two-Liquid-Phase Cultures of Taxus Cells

作　　者：陈海涛[1] 未作君[1] 元英进[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《化学工业与工程》2001年第2期99-102,124,共5页Chemical Industry and Engineering

基　　金：天津市重点攻关资助!项目 ( 90 310 72 11)

摘　　要：基于紫杉醇的光敏性和热敏性特点 ,且以三元萃取方法从两液相培养红豆杉细胞的有机溶剂中提取紫杉醇又不理想的情况下 ,本文提出了有机溶剂 -紫杉醇 -水-甲醇的多元萃取方法 ,并分别测定出甲醇在甲醇 -水 -油酸和甲醇 -水 -混合有机溶剂 (油酸和邻苯二甲酸二丁酯 )休系分配系数变化趋势图 ;分别测定出紫杉醇在甲醇 -水 -油酸和甲醇 -水 -混合有机溶剂体系分配系数变化趋势图。Taxol separation from the organic solvents in two\|liquid\|phase cultures of Taxus cells is a important and necessary step to realize commercial\|scale production of taxol.According to the properties of taxol and the organic solvents,a novel and effective method was proposed for separating taxol from the organic solvents.This method is called multi\|element\|extraction one,of which principles were explained correctly.On the basis of the method,the distribution coefficient of taxol between different aqueous phase and organic phase were obtained through experiments,which are the basis for optimal design of taxol separation technology.

关 键 词：紫杉醇 分离 多元萃取 红豆杉细胞 两液相体系 

分 类 号：TQ28[化学工程—有机化工] TQ028.32