三倍体湘云鲫及其亲本线粒体DNA的比较研究  

Comparative studies on mitochondrial DNA from triploid crucian carp and its parents (allotetraploid hybrids of crucian carp and common carp,♂and Carassius auratus cuvieri,♀)

作　　者：黎双飞[1] 刘少军[1] 张轩杰[1] 罗琛[1] 周工建[1] 刘筠[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《中国水产科学》2001年第1期1-5,共5页Journal of Fishery Sciences of China

基　　金：湖南省自然科学基金重点资助项目!(湘科技字 19973 7)

摘　　要：用人工选育的异源四倍体鲫鲤 (♂ )与白鲫 (♀ )杂交获得具有明显生长优势的三倍体湘云鲫。采用差速离心和核酸酶处理等方法 ,从三倍体、白鲫和异源四倍体鲫鲤肝组织中提取线粒体DNA ,并用 9种限制性内切酶进行单酶酶切分析。经琼脂糖凝胶电泳后计算出各酶切片段的大小 ,测得三倍体、白鲫和异源四倍体鲫鲤mtDNA的分子大小分别为 16 .2 4kb、16 .6 0kb和 16 .2 0kb。根据各单倍型间的酶切片段共享度 ,估算出 3个群体间的遗传距离 ,说明了mtDNA母系遗传的特性。The triploid crucian carp, with obvious advantage in growth, was obtained by crossing allotetraploid hybrids(♂) from offsprings(F 3～F 9)of red crucian carp (Carassius auratus red var.)(♂) ×common carp (Cyprinus carpio L.) (♀), and C.auratus cuvieri (♀). The mitochonodrial DNA (mtDNA) of liver were isolated from the triploids, Japanese crucian carp (C. auratus cuvieri) and allotetraploid hybrids by means of density gradient centrifugation, DNase I and RNase digestion, and were digested by 9 kinds of restriction endonucleases. By analysis of agrose gel electrophoresis, the sizes of mtDNA fragments were estimated that the mtDNA sizes of triploids, C. auratuscuvieri and allotetraploid hybrids were 16.24×10 3, 16.60×10 3 and 16.30×10 3 bp, respectively, and the genetic distances among mtDNA haploidy types in the 3 populations were calculated according to the numbers of common fragments that they had the matrilinear inheritence character of mtDNA.

关 键 词：白鲫 异源四倍体鲫鲤 三倍体湘云鲫 线粒体DNA 限制性内切酶 遗传距离 

分 类 号：S961.6[农业科学—水产养殖] Q959.468[农业科学—水产科学]