桥本甲状腺炎组织中细胞凋亡相关蛋白表达的意义  

Expression of apoptosis-related proteins in Hashimoto′s thyroiditis and its pathological significance

作　　者：张雅萍[1] 姬秋和[1] 张万会[2] 张南雁[1] 宋民喜 陈健康[2] 胡绍文[1] 

机构地区：[1]第四军医大学西京医院内分泌科,西安市710032 [2]第四军医大学生理教研室 [3]西安市第四人民医院内科

出　　处：《中华医学杂志》2001年第7期432-434,共3页National Medical Journal of China

摘　　要：目的　探讨细胞凋亡相关蛋白在桥本甲状腺炎 (HT)发病机制及病理变化中的作用及意义。方法　采用TUNEL法检测HT及非毒性甲状腺肿 (NTG)患者各 17例甲状腺标本中的细胞凋亡水平 ,并用免疫组织化学方法检测凋亡相关蛋白Fas,FasL ,Bcl 2和Bax的表达及分布。结果　HT组细胞凋亡百分率 (32 5 %± 12 3% )比NTG组 (1 3 %± 0 7% )显著增高 (P <0 0 1) ,其甲状腺细胞Fas、FasL、Bcl 2和Bax染色阳性率 (88%～ 94% ) ,也显著高于对照组 (35 %～ 47% ,P <0 0 1)。HT中凋亡细胞及Fas、FasL和Bax染色强阳性甲状腺细胞多分布于浸润淋巴滤泡附近 ,Bcl 2染色强阳性细胞则多分布于远离浸润淋巴滤泡的区域。浸润淋巴细胞中Fas,FasL ,Bcl 2和Bax染色均较弱。结论　HT中有较高水平的细胞凋亡 ,凋亡相关蛋白Fas,FasL ,Bcl 2和Bax在甲状腺细胞中呈特征性分布的高表达 ,而在浸润淋巴细胞中表达较少 ,这些改变与甲状腺滤泡萎缩、破坏及弥漫性淋巴细胞浸润有关。Objective To explore the role and significance of apoptosis related proteins in the pathogenesis and pathological changes in Hashimoto′s thyroiditis(HT). Methods Apoptotic level in thyroid tissue specimens from 17 patients with HT and 17 patients with nontoxic goiter (NTG) was evaluated by TUNEL method. Expression and distribution of Fas, FasL, Bcl 2 and Bax proteins in the specimens were investigated with immunohistochemical methods. Results A high percentage of apoptosis (32.5%±12 3%) was observed by TUNEL method in thyroid follicles from HT specimens in comparison with that from NTG specimens(1.3%±0.7%, P <0.01). Positive rates of Fas, FasL, Bcl 2 and Bax in thyroid cells of NTG(35%～47%)were significantly lower than those in thyroid cells of HT(88%～94%, P <0.01). The intensity of positive immunostaining for Fas, FasL, Bcl 2 and Bax in thyroid cells of HT was significantly higher than that of the controls( P <0.01). Apoptosis and strongly positive immunostained thyroid cells with HT for Fas, FasL and Bax were mainly distributed in follicles adjacent to lymphocytic infiltrates, whereas strongly positive stained thyroid cells for Bcl 2 were mainly located in the area remote from the infiltrating lymphocytes. Staining of infiltrating lymphocytes for Fas, FasL, Bcl 2 and Bax was weak. Conclusion Thyroid follicles in HT specimens exhibited high apoptotic levels. The high expression of apoptosis related proteins Fas, FasL, Bcl 2 and Bax in thyrocytes of HT and weak expression in infiltrating lymphocytes accounted for thyroid follicle destruction and diffuse lymphocytic infiltration in HT.

关 键 词：桥本甲状腺炎 细胞凋亡相关蛋白 发病机制 病理 

分 类 号：R581.4[医药卫生—内分泌]