同型半胱氨酸对内皮细胞分泌组织型纤溶酶原激活物及其抑制剂-1的影响  

The Effects of Homocysteine on Endothelial Cell Secreting Tissue-Type Plasminogen Activator and its Inhibitor-1

作　　者：薛冠华[1] 张纪蔚[1] 张柏根[1] 袁静[2] 

机构地区：[1]上海第二医科大学附属仁济医院血管外科,上海市200001 [2]上海第二医科大学附属仁济医院药剂科,上海市200001

出　　处：《中国循环杂志》2001年第2期144-146,共3页Chinese Circulation Journal

摘　　要：目的：研究同型半胱氨酸（Ｈｃｙ）对内皮细胞分泌组织型纤溶酶原激活物（ｔ－ＰＡ）和纤溶酶原激活物抑制剂－ｌ（ＰＡＩ－１）的影响。 方法：培养内皮细胞，分实验组和对照组，各组均为６孔细胞，每孔内皮细胞数为每毫升２．５×１０５个细胞。加入Ｈｃｙ，测定不同浓度不同时间细胞培养液中ｔ－ＰＡ和ＰＡＩ－１的含量。 结果：Ｈｃｙ可引起内皮细胞形态的损伤，并使其分泌ｔ－ＰＡ和 ＰＡＩ－１在１ｍｍｏｌ实验组、２．５ ｍｍｏｌ实验组作用 ２４小时后与对照组比明显升高，有显著差异（Ｐ＜０．０５），且 ５ ｍｍｏｌ实验组Ｈｃｙ作用 ４、８小时后与对照组比明显升高，有显著差异（Ｐ＜０．０５）。 结论：Ｈｃｙ影响内皮细胞分泌ｔ－ＰＡ与ＰＡＩ－１的平衡可能是动脉粥样硬化的发病机制之一。Objective:To study the effects of homocysteine(Hcy) on endothelial cell secreting tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor(PAI-1). Methods:Cultured endothelial cell was exposed to Hey at the concentration of 0, 1 , 2. 5, 5 mmol for 2, 4, 8, 12 and 24 h respectively,in the end of each experiments,the morphological changes were observed and medium was collected to assay the concentration of t-PA and PAl-l. Results:Hcy may do an injury in the morphology of cultured endothelial cell. Endothelial cells' secreting t-PA and PAl-l were significantly increased in 1 mmol and 2. 5 mmol groups after 24 hours than those in o mmol group (p< 0. 05),and remarkably improved in 5 mmol group with 4 and 8 hours than those in o hour group(p<0. 05). Conclusion:Endothelial cell secreting t-PA and PAl-l imbalance may be one of the atherogenic mechanism of Hcy.

关 键 词：半胱氨酸 内皮细胞 组织型纤溶酶原激活物 动脉粥样硬化 PAI-1 

分 类 号：R543.5[医药卫生—心血管疾病]