提高人血丙种球蛋白制品稳定性方法的研究  

Study on stability of human serum γ-globulin products

作　　者：王期中 张顺龙 李西河 徐贯芬 

机构地区：[1]山东省生物制品研究所,山东泰安271000

出　　处：《中国生化药物杂志》2001年第1期23-24,共2页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究解决人血丙种球蛋白制品因纤溶酶原及纤溶酶引起的IgG裂解问题 ,以提高该制品的稳定性。方法采用Sepharose 4B与L 赖氨酸偶联后吸附人血丙种球蛋白制品中纤溶酶原及纤溶酶。 结果被吸附过的制品与未被吸附过的制品相比较 ,其裂解程度显著降低。结论在人血丙种球蛋白生产中 ,加入L 赖氨酸 Sepharose 4B吸附纤溶酶原和纤溶酶操作步骤可明显提高产品稳定性 ,是提高产品质量的有效措施。PurposeThe aim is to increase stability and to solve the problem of splitting of human serum γ globulin products.MethodsFibrinolysin and profibrinolysin in the human serum γ globulin products were absorbed with L lysine Sepharose 4B.ResultsCompared with the unabsorbed products, the splitting degree of the absorbed products decreased obviously.ConclusionIn manufacture of human serum γ globulin products,absorbing fibrinolysin and profibrinolysin with L lysine Sepharose 4B may increase stability of the product.It was an effective method to improve the quality of product.

关 键 词：人血丙种球蛋白 SEPHAROSE 4B 纤溶酶 纤溶酶原 稳定性 

分 类 号：R392－33[医药卫生—免疫学] R973[医药卫生—基础医学]