抗体预处理对胰岛移植物的影响  

The influence of pretreatment with Anti-B lymphocyte serum on immunogenecity of islet preparations

作　　者：董维平[1] 张洪德[1] 王煜非[1] 

机构地区：[1]上海市第一人民医院糖尿病研究室,200080

出　　处：《中国糖尿病杂志》2001年第2期76-78,共3页Chinese Journal of Diabetes

摘　　要：目的　研究抗猪 B淋巴细胞抗血清预处理胰岛制备物对其免疫原性和功能的影响。方法　用混合成年猪 B淋巴细胞为抗原制备抗血清 ,预处理猪 ICCs。以胰岛 -淋巴细胞混合培养和胰岛内 SL A- DR阳性细胞数检测胰岛的免疫原性 ;以胰岛素释放试验检测胰岛功能。结果　经抗猪 B淋巴细胞抗血清预处理组胰岛 -淋巴细胞混合培养的 cpm值、MHCI类抗原和 SL A- DR抗原阳性细胞数较对照组均明显降低 ,有非常显著性差异 (P<0 .0 0 1) ;胰岛素释放值无明显差异。结论　应用抗猪 B淋巴细胞血清预处理能降低胰岛制备物的免疫原性 。Objective To investigate the influence of pretreatment with anti-B lymphocyte serum on the immunogenecity and endocrine function of islet preparations.Methods Porcine ICCs were pretreated with anti-B lymphocyte serum which was produced using the mixed adult porcine B lymphocyte as antigen.The immunogenecity of islet was measured by mixed islet lymphocyte culture (MILC) and SLA-DR positive cell number within the islet and the islet function was assayed with insulin release test.Results The cpm value of MILC of the group pretreated with anti-B lymphocyte serum and the MHCⅠ antigen and SLA-DR antigen positive cell numbers was markedly decreased in comparison with that of control group ( P <0.001).There was no significant difference in insulin release of additional different groups.Conclusion The pretreatment of islet preparation with anti-B lymphocyte serum may decrease the immunogenecity of islet preparation,while there is no marked influence on its insulin release function.

关 键 词：胰岛 移植物 预处理 糖尿病 治疗 

分 类 号：R587[医药卫生—内分泌]