几种肝脏疾病血清中糖基化磷脂酰肌醇特异性磷脂酶D的活性改变  

Changes of Serum Glycosylphosphatidylinositol Specific Phospholipase D Activity in Patients of Several Liver Diseases

作　　者：唐建华[1] 李文凯[1] 谢玉桃[1] 罗秀菊[1] 顾善兰[1] 彭小玲[1] 谢妮[1] 

机构地区：[1]中南大学湘雅医学院,湖南长沙410078

出　　处：《中国医师杂志》2001年第4期253-255,共3页Journal of Chinese Physician

基　　金：国家自然科学基金资助课题! (39970 31 5);湖南省卫生厅资助课题! (980 8)

摘　　要：目的　观测几种肝脏疾病患者血清中糖基化磷脂酸肌醇特异性磷脂酶D(GPI -PLD)的活性改变。方法　利用自制的具完整糖基化磷脂酰肌醇 (GPI)结构的胎盘型碱性磷酸酶 (PLAP)做底物 ,通过TX - 114分相 ,定量测定血清GPI-PLD的活性 ,用SPSS 10 0统计软件分析资料。结果　检测 172例肝脏疾病患者 (包括A组 :2 6例急性重症病毒性肝炎 ;B组 :2 9例肝硬化 ;C组 :32例慢性病毒性肝炎 ;D组 :55例急性非重症病毒性肝炎 ;E组 :30例原发性肝癌 )血清中GPI -PLD的活性 (转化底物的百分率 ) ,发现A、B两组患者的该酶活性较正常人 (182名 )显著性降低 ,而D、E两组则较正常人显著性增高 ,C组患者的酶活性与正常人无显著性差异 ,但A、B、C、D、E两组之间的酶活性水平改变却均有显著性。结论　检测患者血清GPI -PLD活性水平 ,既可作为临床诊断急性病毒性肝炎、肝硬化、原发性肝癌等肝脏疾病的一项生化指标 ,也可作为这些疾病临床疗效和预后的一项判断指标。Objective To determine the changes of serum glycosylphosphatidylinositol specific phospholipase D(GPI-PLD) activity in patients of several liver disease.Method Glycosylphosphatidylinositol (GPI) anchored placental alkaline phosphatase(PLAP) prepared by ourselves was used as a substrate.After partitioning by triton-X-114,the serum GPI-PLD activity was determined quantitatively and the data was treated by microware of SPSS 10.0.Results On the basis of the percentage of GPI-anchored PLAP conversion,the sera GPI-PLD activities of total 172 patients,included 26 severe acute viral hepatitis as group A,29 liver cirrhosis as group B,32 chronic viral hepatitis as group C,55 mild acute viral hepatitis as group D,30 primary hepatocellular carcinoma as group E,were measured.As compared with 182 healthy presons as control group,the sera GPI-PLD activities of group A and B were significantly reduced;By contraries,the activities of group D and E were significantly raised.The sera GPI-PLD activities of group C compared with healthy control group were not significantly altered.However,when paired Q-test,the changes of serum GPI-PLD activity between all paired groups among this five groups were remarkable.Conclusions The determination of sera GPI-PLD activities in patients can act as a biochemistry index for diagnosis of acute viral hepatitis,liver cirrhosis and primary hepatocellular carcinoma,as well as an auxiliary index for judgment of the curative effect and prognosis of liver diseases.

关 键 词：肝疾病 血清 磷脂酶D GPI-PLD PLAP 

分 类 号：R446.112[医药卫生—诊断学] R575[医药卫生—临床医学]