日本血吸虫快速酶联免疫吸附试验抗体检测试剂盒的应用研究  

STUDY ON THE APPLICATION OF ANTIBODY DETECTING FAST ELISA (F-ELISA) KIT FOR IMMUNODIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

作　　者：张顺科[1] 曾宪芳[1] 易新元[1] 李忠杰[1] 汪世平[1] 曾庆仁[1] 

机构地区：[1]湖南医科大学寄生虫学教研室,长沙410078

出　　处：《中国血吸虫病防治杂志》2001年第2期79-81,共3页Chinese Journal of Schistosomiasis Control

摘　　要：目的　探讨日本血吸虫快速酶联免疫吸附试验 (F- EL ISA )抗体检测试剂盒检测日本血吸虫病的特异性、敏感性及与其他寄生虫病的交叉反应。方法　采用 F- EL ISA法检测日本血吸虫急性、慢性及晚期病人血清中抗 SEA抗体和健康人、并殖吸虫、肝吸虫、囊虫及旋毛虫病患者血清中的交叉抗体。结果　检测急性、慢性及晚期血吸虫病人的敏感性分别为 10 0 %、95 .19%和 77.78% ,检测正常人的特异性为 98.46 % ,与并殖吸虫、肝吸虫、囊虫及旋毛虫病人的交叉反应率分别为11.6 3%、7.5 5 %、0和 13.33%。结论　 F- EL ISA检测日本血吸虫病具有敏感性高、特异性强、快速简便等优点 。Objectives To elucidate the specificity and sensitivity of F ELISA (Fast enzyme linked immunosorbent assay) kit for immunodiagnosis of schistosomiasis japonica and its cross reactivity with other parasites. [WT5”HZ]Methods [WT5”BZ]Anti SEA antibodies in sera from the patients with schistosomiasis japonica and the cross reactivity with sera from normal presons and the patients with other parasites were detected by F ELISA. Results The sensitivities of F ELISA for acute, chronic and advanced schistosomiasis japonica were 100%, 95 19% and 77 78% respectively. The specificity of F ELISA for normal persons was 98 46%. The cross reaction rate with paragonimiasis, clonorchiasis, cysticercosis and trichinelliasis were 11 63%, 7 55%, 0 and 13 33% respectively. [WT5”HZ]Conclusion [WT5”BZ]The F ELISA kit shows high specificity and sensitivity and has advantages of simplicity and high speed, and thus is of higher value in immunodiagnosis of schistosomiasis japonica.

关 键 词：日本血吸虫 F-ELISA 抗体 检测 F-ELISA 

分 类 号：R532.21[医药卫生—内科学] R383.24[医药卫生—临床医学]