四君子汤复方总多糖对小鼠肠道粘膜相关淋巴组织的影响  

Influence of total polysaccharide extracted from Sijunzi decoction on the intestinal mucosa associated lymphoid tissues of mice

作　　者：刘良[1] 周华[2] 王培训[2] 胡英杰[2] 

机构地区：[1]香港浸会大学 [2]广州中医药大学,广州510405

出　　处：《中国免疫学杂志》2001年第4期204-206,F003,共4页Chinese Journal of Immunology

基　　金：国家"九五"攀登计划预选项目!( 980 0 8)

摘　　要：目的 :通过环磷酰胺所致小鼠肠道粘膜相关淋巴组织损伤模型研究四君子汤复方总多糖对肠道粘膜免疫的影响。方法 :观察口服 1g kg四君子汤复方总多糖对 10 0mg kg的环磷酰胺处理 2 4h后小鼠Peyer’s结的数目、Peyer’s结内细胞总数、CD3+ 、IgA+ 细胞数量和细胞凋亡的情况。结果 :四君子汤复方总多糖可对抗环磷酰胺诱导的小鼠肠道粘膜相关淋巴组织中Peyer’s结数目、Peyer’s结细胞总数、肠粘膜内CD3+ 、IgA+ 细胞量的减少 ,细胞凋亡程度也明显减轻。结论 :四君子汤复方总多糖可对抗环磷酰胺诱导的小鼠肠粘膜相关淋巴组织损伤 ,提示多糖可能对肠道粘膜免疫具有改善作用。Objective:By using the model of intestinal mucosal associated lymphoid tissue injury by cyclophosphamide, to understand the influence of total polysaccharide extracted from Sijunzi decoction (TPSJ) on the intestinal mucosal associated lymphoid tissue which reflect the status of the intestinal mucosal immune of mice Methods:Investigate the effects of TPSJ to the number of Peyer's patches of mice, total cell number of Peyer's patches, the number of CD3 + and IgA + cell, apoptosis at 24 h after being treated with 100 mg/kg cyclophosphamide Results:TPSJ could increase the number of Peyer's patches of mice, total cell number of Peyer's patches, the number of CD3 + and IgA + cell, and could reduce apoptosis as well Conclusion:TPSJ could antagonize the injury of intestinal mucosal associated lymphoid tissues of mice induced by cyclophosphamide, suggesting that this polysaccharide can improve the intestinal mucosal immunofunction

关 键 词：四君子汤 肠道粘膜免疫 环磷酰胺 复方总多糖 中药 小鼠 

分 类 号：R285.5[医药卫生—中药学]