环磷酰胺对小鼠Peyer’s结和肠道粘膜相关淋巴细胞的影响  

The influnence of cyclophosphamide on Peyper's patches and intestinal mucosal associated lymphocytes in mice

作　　者：周华[1] 王培训[1] 刘良[2] 周联[1] 

机构地区：[1]广州中医药大学,广州510405 [2]香港浸会大学

出　　处：《中国免疫学杂志》2001年第4期186-187,189,F002,共4页Chinese Journal of Immunology

基　　金：国家"九五"攀登计划预选项目!( 980 0 8)

摘　　要：目的 :研究环磷酰胺对Peyer’s结和肠道粘膜相关淋巴细胞的影响 ,建立肠道粘膜免疫障碍模型。方法 :剂量为10 0mg kg的环磷酰胺 ,观察不同时间处理后小鼠Peyer’s结的数目以及处理 2 4h时Peyer’s结内细胞总数、CD3+ 、IgA+ 细胞数量和细胞凋亡的情况。结果 :环磷酰胺可使Peyer’s结数目在 2 4h时降低最明显 ,结内细胞数量和CD3+ 、IgA+ 细胞也明显减少 ,而细胞凋亡明显增加。结论 :环磷酰胺可诱导肠粘膜免疫障碍 ,这可能是其引起淋巴细胞的细胞凋亡而产生的。Objective:To understand the influence of cyclophosphamide to Peyer's patches and intestinal mucosal associated lymphocytes and establish an intestinal mucosal immune dysfunction model Methods:Investigate the number of Peyer's patches of mice at different time points and the total cell number of Peyer's patches, the number of CD3 + and IgA + cell, apoptosis at 24 h after being treated with 100 mg/kg cyclophosphamide Results:Cyclophosphamide could induce markedly decreasing of Peyer's patches at 24 h after treatment, at which the total cell number of Peyer's patches, the number of CD3 + and IgA + cell decreased significantly, and the apoptosis increased obviously Conclusion:Cyclophosphamide can induce the dysfunction of intestinal mucosal immune in mice, which is maybe mediated by the apoptosis of lymphocytes

关 键 词：环磷酰胺 肠道粘膜 淋巴细胞 影响 Peyer's结 抗癌药 免疫障碍 

分 类 号：R979.1[医药卫生—药品] R967[医药卫生—药学]