RAPD标记在桉属种间杂交一代的分离方式研究  

Segregation of RAPD Markers: A Case in Interspecific Cross of Eucalyptus

作　　者：甘四明[1] 施季森[2] 白嘉雨[1] 吴坤明[1] 吴菊英[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]南京林业大学林木遗传与基因工程实验室,江苏南京210037

出　　处：《林业科学研究》2001年第2期125-130,共6页Forest Research

基　　金：国家自然科学基金项目!( 3 9870 61 9);国际科学基金项目!( International Foundation for Science;IFS) ( D/2 94 7-1 )

摘　　要：以 1个尾叶桉×细叶桉全同胞家系的 2个亲本和 2 12个子代为材料 ,利用 RAPD分子标记技术进行了 RAPD标记在桉树中的分离方式研究。结果表明 :7个随机引物共扩增出 4 0个标记 ,标记在 F1代的分离方式可以分为两类 :符合孟德尔方式和偏离孟德尔方式。符合孟德尔方式的标记包括 :亲本均有而在子代中不分离的 9个 (在这类位点上代表的杂交组合为 AA× AA、AA× Aa或 Aa× AA) ,亲本间呈多态性而在子代中不分离的 7个 (aa× AA或 AA× aa) ,亲本间均有而在子代中 3∶ 1分离的 2个 (Aa× Aa) ,亲本间呈多态性且在子代中 1∶ 1分离的 9个 (Aa× aa或 aa×Aa)。偏分离的标记包括 :亲本间呈多态性而在子代中偏离 1∶ 1分离的 12个 ,两亲本均有而在子代中偏离 3∶ 1分离的 1个。亲本间呈多态性且在子代中 1∶ 1分离的位点在拟测交策略中可以用于遗传连锁图谱构建 ,这为利用 RAPD标记构建桉树遗传连锁图谱提供了理论支持。Seven 10 mer primers were used for analysis on segregation of RAPD (Random amplified polymorphic DNA) markers in an interspecific F 1 population of Eucalyptus urophylla × E. tereticornis, including 2 parents and 212 sibs. Of the total 40 fragments amplified, 28 (70%) were polymorphic between parents and 24 (60%) segregant in the sibs, indicating a high level of polymorphism and heterozygosity at RAPD loci between the two parents. There were 27 markers segregating in a Mendelian pattern, including 9 markers presenting in both parents and all sibs (representing a genotypic combination AA×AA, AA×Aa or Aa×AA),7 markers polymorphic between the parents but homologous in all sibs (AA×aa or aa×AA),2 markers presenting in both parents and segregating in the sibs in a ratio 3∶1 (Aa×Aa), and 9 markers polymorphic between the parents and segregating in the sibs in a ratio 1∶1 (Aa×aa or aa×Aa). There were 13 markers segregating in a nonmendelian pattern, including 12 markers polymorphic between parents but segregating in the sibs in a ratio distortive from 1∶1, and 1 markers homologous in both parents but segregating in the sibs in a ratio distortive from 3∶1. Those markers that were polymorphic between parents and segregating 1∶1 in the sibs, following a pseudo testcross configuration, could be potentially useful for genetic linkage map construction of the representative species, which would lay a theoretical basis for genetic linkage map construction using RAPD markers and F 1 population.

关 键 词：RAPD标记 桉树属 种间杂交 杂种一代 分离方式 遗传连锁图谱 

分 类 号：S792.390.4[农业科学—林木遗传育种] S722.3[农业科学—林学]