肌肉电转促血管形成素-1基因治疗大鼠下肢血管闭塞病  

Intramuscular electroporation of angiopoietin-1 gene augments revascularization in the rat ischemic hindlimb

作　　者：姜宁[1] 高炜[1] 王日胜[1] 朱建健[1] 郭艳红[1] 王建[1] 沈冬[2] 朱国英[1] 陈光慧[2] 汤健[2] 

机构地区：[1]北京大学第一医院心内科,100034 [2]北京大学心血管研究所

出　　处：《中国介入心脏病学杂志》2001年第2期101-103,共3页Chinese Journal of Interventional Cardiology

摘　　要：目的　　观察肌肉电转促血管形成素－１（ａｎｇｉｏｐｏｉｅｔｉｎ－１，Ａｎｇ－１）基因对大鼠下肢血管闭塞病的治疗效果。方法　　制作大鼠下肢血管闭塞病模型，通过电转Ａｎｇ－１进行基因治疗，以ＲＴ－ＰＣＲ及免疫组化方法观察　Ａｎｇ－１基因的表达，应用微血管计数、血管造影技术及相关临床指标观察　Ａｎｇ－１基因导入大鼠体内的生物学效应。结果　　（１）肌肉电转ｐｃＤ２Ａｎｇ－１基因可在大鼠局部转基因肌肉组织高效表达Ａｎｇ－１；（２）转ｐｃＤ２Ａｎｇ－１基因组大鼠下肢肌肉组织新生血管及侧支循环数目明显多于同期空载质粒对照组。结论　　转Ａｎｇ－１基因可促进大鼠局部组织新生血管形成和侧支循环建立，恢复闭塞部位血供，从而有效治疗血管闭塞病。Objective We tested the hypothesis that gene transfer of plasmid DNA encoding angiopietin- 1(Ang-1) could modulate collateral vessel development in a rat model of hindlimb ischemia. Methods PcD_2 and pcD_2 Ang-l were electroporated intramuscularly into the rat ischemic hindlimb. Couateral vessel development and limb perfusion were assessed by capillary density, capillary / muscle fiber ratio, angiograghy and clinical observation. Results (1) The expression of Ang-1 gene was comfirmed RT-PCR and immunohistochemistry;(2) Collateral vessels developed more with pcD_2/Ang-1 than those with pcD_2. Conclusion Intramuscular electroporation of Ang-1 gene produces anatomic and physiological evidence of enhanced collateral vessels formation. Ang-1 may modulate neovascularization in adult animals and thus represents a feasible therapeutic strategy for patients with tissue ischemia.

关 键 词：促血管形成素-1 基因治疗 血管闭塞病 

分 类 号：R543.05[医药卫生—心血管疾病]