氟中毒大鼠睾丸细胞一氧化氮合酶活性变化的形态计量学研究  

Effect of fluorosis on nitric oxide synthase activity of rat testis:a morphometric study

作　　者：李积胜[1] 汪超[1] 王静[2] 罗志勇[1] 何伟[1] 

机构地区：[1]武警医学院解剖学教研室,天津300162 [2]西安医科大学公共卫生学院

出　　处：《武警医学》2001年第3期143-145,共3页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金!经费资助 (No .3 9870 689)

摘　　要：目的　探讨氟中毒对睾丸结构和功能损害的分子机制 ,研究其对睾丸一氧化氮合酶 (NOS)活性的影响。方法　选用 2月龄Wistar雄性大鼠 1 6只 ,均分为实验组和对照组 ,实验组饮用含 2 0 0mg/L氟化钠的去离子水 ,对照组单纯饮用去离子水。 1 2周后 ,采用NADPH -d组织化学法显示睾丸细胞NOS活性。结果　与对照组相比 ,氟中毒大鼠睾丸间质细胞NOS阳性细胞数目明显增多 (P <0 0 1 ) ,NOS活性明显增强。结论　氟中毒大鼠睾丸细胞NOS活性明显增强 。Objective To study the changes of testis nitric okide synthase (NOS) activity in male rats with fluorosis,thereby promoting the understanding of the molecular mechanism of injurious effect of fluorosis on the development and function of testis.Methods Sixteen Wistar male rats were divided into 2 groups of 8 each:fluorosis rats and control rats.Fluorosis rats were exposed to sodiam fluoride(200 mg/L) in their drinking water for 12 weeks,after which time the testis NOS was nssayed by NADPH-d histochemistry.Results Compared with the control group,fluorosis caused increase in festis NOS activity as observed under light microscope.Conclusions Fluorosis can increase NOS positive cells (P<0.01) and NOS activity in festes,which might be one of the molecular mechanisms of the injurious action of fluorosis on testis development and function.

关 键 词：氟中毒 睾丸 一氧化氮合酶 

分 类 号：R599.9[医药卫生—内科学] R339.21[医药卫生—临床医学]