棒状链霉菌甘油耐受量与克拉维酸合成的关系  

Relationship on glycerol tolerant and clavulanic acid synthesis in Streptomyces clavuligerus

作　　者：李丹丹[1] 黄喜桂[1] 林文良[1] 程元荣[1] 

机构地区：[1]福建省微生物研究所

出　　处：《中国抗生素杂志》1999年第1期14-15,共2页Chinese Journal of Antibiotics

摘　　要：在克拉维酸产生菌的菌种选育过程中，应用甘油耐受量作为选择压力能有效地除去低效价菌株，提高高产变株的检出率，较传统筛选法优越。试验表明，甘油耐受量高的菌株克拉维酸效价也高。在高甘油含量（１１％）的甘油－精氨酸琼脂（ＡＧＳ）平板挑取的菌株基本上无负变株，与对照菌株相比，有的菌侏效价提高２０％～３０％。耐甘油量低（６％）的变株，其效价比对照菌株降低了９５％。During selection of clavulanic acid producing strain, using glycerol tolerant as selection pressure can obviate low producing strain and increasing the successful rate of obtaining hith producing strain, it is better than traditional screening approach. Experiment showed that the higher the glycerol tolerant, the higher clavulanic titre is. The strains that come from AGS plate containing 11 per cent of glycerol were generally no negative mutation and clavulanic production was 21 30 per cent higher than mother strain. In the other hand, the production in low glycerol tolerant (6 per cent) mutation strain was 95 per cent less than the mother strain.

关 键 词：棒状链霉菌 克拉维酸 生物合成 甘油 

分 类 号：R978.11[医药卫生—药品] TQ465[医药卫生—药学]