神经肽Y和一氧化氮合酶阳性神经元在大鼠大脑皮质枕叶的分布及其衰老变化  

Distribution and aged-related change of neuropeptide Y and nitric oxide synthase-positive neurons in occipital cortex of rats

作　　者：李秋明[1] 王荣华[1] 田秀珍[2] 

机构地区：[1]哈尔滨医科大学组胚教研室,黑龙江哈尔滨150086 [2]哈尔滨理工大学卫生院,黑龙江哈尔滨150080

出　　处：《哈尔滨医科大学学报》2001年第2期100-102,F003,共4页Journal of Harbin Medical University

摘　　要：目的　研究大鼠皮质枕叶神经肽Y(NPY)和一氧化氮合酶 (NOS1)阳性神经元的分布及其衰老变化。方法　采用免疫组化SABC法 ,观察 3个不同年龄组大鼠大脑皮质枕叶NPY和NOS1阳性神经元的分布及形态特征 ,并进行比较。结果　NPY和NOS1阳性细胞在皮质Ⅱ～Ⅵ层均有分布 ,两者都属于非锥体形神经元 ;比较 3个年龄组两种阳性细胞的数量和截面积的变化 ,发现老年组与幼年组及中年组相比 ,两项指标都显著减少 (P <0 .0 1)。结论　NPY和一氧化氮 (NO)作为神经递质 ,可能与大脑皮质枕叶功能的调节有关 ;Objective To study distribution and aged related change of neuropeptide Y and nitric oxide synthase(NOS) positive neurons in occipital cortex of rats.Methods Immunohistochemical SABC method was used to observe and compare distribution and morphological character of neuropeptide Y and NOS positive neurons in occipital cortex of 3 groups rats.Results NOS 1 and NPY positive neurons distributed in layers Ⅱ～Ⅵ of cerebral cortex,and both of them were shown non pyramidal form;comparing the old group with the infant and the adult,the number and section acreage of positive cells had significant decrease( P <0.01).Conclusion As neurotransmitter,NPY and nitric oxide(NO) is probably related to the regulation function of occipical cortex,NPY and NOS 1 positive neurons changes with aging,which influence natural physiological function of NPY and NO.

关 键 词：一氧化氮合酶 神经肽Y 大鼠 大脑皮质 衰老 免疫组织化学 阳性神经元 

分 类 号：R339.38[医药卫生—人体生理学] R338[医药卫生—基础医学]