救脑宁注射液对神经细胞凋亡及胞浆钙变化的影响  

Effect of Jiunaoning Injection on apoptosis and changes in cytosolic calcium induced by hypoxia / hypoglycemia in SH-SY5Y human neuroblastoma cells

作　　者：何丽云[1] 范吉平[1] 孙塑伦 黄启福[2] 王硕仁[1] 朱陵群[1] 张壮[1] 崔巍[1] 高颖[1] 邹忆怀[1] 

机构地区：[1]北京中医药大学东直门医院脑病研究室,北京100700 [2]国家中医药管理局,北京100010

出　　处：《中国病理生理杂志》2001年第4期312-314,共3页Chinese Journal of Pathophysiology

基　　金：国家九五攻关课题!资助 (NO :96 - 90 6 - 0 6 - 0 1)

摘　　要：目的 :研究神经细胞经缺氧 缺糖培养诱导的凋亡发生、胞内钙离子变化及救脑宁注射液的治疗作用。方法 :碘化丙啶染色 ,流式细胞仪定量分析细胞凋亡百分率 ;Fluo - 3负载 ,流式细胞仪检测胞内钙平均荧光值变化。结果 :缺氧 缺糖培养可诱导神经细胞凋亡和引起细胞内钙离子增高。救脑宁注射液能抑制细胞凋亡、降低细胞内游离钙离子浓度。结论 :救脑宁注射液能抑制缺氧 缺糖诱导的神经细胞凋亡及钙超载 ,有神经细胞保护作用。AIM: To study the effect of Jiunaoning Injection on apoptosis and the changes in cytosolic calcium induced by hypoxia/hypoglycemia in SH-SY5Y human neuroblastoma cells. METHODS: The apoptosis and the changes in cytosolic calcium were induced by hypoxia and hypoglycemia .Apoptosis was measured via flow cytometry(FCM), and cytosolic calcium was determined by Fluo-3 fluorometry. RESULTS: The hypoxia/hypoglycemia induced apoptosis and increased the cytosolic calcium in SH-SY5Y cells. Jiunaoning Injection inhibited the apoptosis of the SH-SY5Y cells and decreased the concentration of cytosolic free calcium. CONCLUSION: Hypoxia/hypoglycemia induced apoptosis and increase in cytosolic free calcium in SH-SY5Y cells, which were significantly inhibited by Jiunaoning Injection.

关 键 词：中草药 细胞凋亡 救脑宁注射液 缺氧/缺糖 神经细胞 胞浆钙 

分 类 号：R277.733.1[医药卫生—中医学] R285.5