基体辅助激光解吸电离质谱法对DNA样品的高分辨率测定  

High Resolution Matrixassisted Laser Desorption/IonizationTimeofflight Mass Spectrometry Analysis of DNA

作　　者：邓慧敏[1] 黎军[1] 赖志辉 查庆民 赵善楷 

机构地区：[1]中山大学测试中心

出　　处：《高等学校化学学报》1999年第6期957-959,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：基体辅助激光解吸电离飞行时间质谱（ＭＡＬＤＩ－ＴＯＦ－ＭＳ）法因其具有测定质量范围大、灵敏度高、速度快及精确度好等优点，近年来已成为测定多肽、蛋白质、核酸、多糖等生物大分子分子量及其一级结构的有力工具．此外，ＭＡＬＤＩ－ＴＯＦ－ＭＳ法还可用于Ｃ６０、...High resolution mass spectra of DNA samples were acquired by using matrixassisted laser desorption/ionization timeofflight mass spectrometry(MALDITOFbyMS) using mixture of 3hydroxypiclinic acid and picnilic acid as a matrix. The resolutions of d(C)10, d(G)10, d(T)18, and \{d(TTACTCTGTTAATGTCTTTG)\} monoisotope peaks have reached \{5 513\}, \{3 895\}, \{10 468\} and \{8 769\} respectively. The experiment results showed that the DNA samples with a higher purity and stability could get higher resolutions. C18 SepPak cartridge, NH+4 cationexchange resin and nitrocellulose(NC) film substrate have been applied to eliminating Na+, K+ ions and other impurities from DNA syntheses, and to purifying the analytes and obtaining high resolution mass spectra with enhanced sensitivity and reproducibility.

关 键 词：基体辅助激光解吸电离飞行时间质谱法 高分辨率质谱图 DNA 测定 

分 类 号：Q523[生物学—生物化学]