血红素在有序分子膜中的行为研究  

The Investigation of Behavior of Heme in Ordered Molecular Films

作　　者：欧阳健明[1] 

机构地区：[1]暨南大学化学系,广州510632

出　　处：《无机化学学报》2001年第3期349-354,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金重点项目(No.20031010);教育部重点科学技术项目(1998-121);广东省自然科学基金(No.9

摘　　要：本文研究了血红素（Ｈｅｍｅ）在硬脂酸（ＳＡ）单分子膜和Ｌａｎｇｍｕｉｒ－Ｂｌｏｄｇｅｔｔ（ＬＢ）膜中的行为。Ｈｅｍｅ及其与ＳＡ的混合物均能在纯水亚相形成稳定的单分子膜。当摩尔分数ｘＨｅｍｅ＜０．３时，Ｈｅｍｅ以“面接触亚相”的方式平躺在 ＳＡ所形成的单分子膜中；而当 ｘＨｅｍｅ≥ ０． ３时， Ｈｅｍｅ以其“边接触亚相”的方式自身成膜。通过在不同表面压和不同摩尔比下沉积的Ｈｅｍｅ－ＳＡ混合ＬＢ膜的紫外－可见光谱、偏振紫外一可见光谱、小角Ｘ－射线衍射和荧光光谱，表征了血红素在有序分子膜中的排列取向和形态，为日后进一步研究血红素在生物系统中的化学物理机制提供了有价值的信息。The monolayer formation and the deposition of Langmuir-Blodgett films of the mixtures of Heme and stearic acid with different molar ratio were investigated. The result shows that they can form stable monolayer on pure water subphase. When x(Heme) < 0. 3, Heme lies on a 'face on ' position in the monolayer. When x(Heme)greater than or equal to0. 3, Heme lies on an ledge on position. The Langmuir-Blodgett (LB) films of Heme and its mixtures with stearic acid were deposited on the hydrophilic quartz plates. Some information about the orientation of Heme in LB films were obtained from the UV-visible and polarized UV-visible spectra, low angle X-ray diffraction, and fluorescent spectra. The absorbance of the mixed LB films of Heme-SA is greatly increased with the increase of deposition pressure or the molar fraction of Heme. Although the increase of absorbance may be resulted from the increase of the Heme concentration in LB films at higher surface pressure, most of the increase was resulted from the ordered arrangement of Heme molecules. These results provide a good guidance for the further research and applications of Heme derivatives.

关 键 词：血红素 混合单分子膜 有序分子膜 混合LB膜 硬脂肪 Hame 

分 类 号：O484[理学—固体物理]