细胞显微光谱研究竹红菌甲素在癌细胞内的滞留效应  

Study on the Detaining Effect of Hypocrelin A in the Cancer Cell Using Microspectrum

作　　者：俞帆[1] 黄守江[1] 龚光林[1] 曾昭权[1] 李玛琳[2] 

机构地区：[1]云南大学实验中心,昆明650091 [2]昆明医学院药理重点实验室,昆明650031

出　　处：《光谱学与光谱分析》2001年第2期227-229,共3页Spectroscopy and Spectral Analysis

基　　金：云南省教委的云南省高等学校联合测试基金

摘　　要：利用细胞显微光谱技术 ,在 2 0 0 0 0～ 12 0 0 0cm- 1 范围内测量了经过浓度为 2 0 μg·mL- 1 竹红菌甲素(HA)培养 2 3个小时的小鼠淋巴癌细胞和未经HA培养的小鼠淋巴癌细胞的光谱。结果显示两者有明显的差异 ,说明HA使癌细胞发生了改变 ;在经HA培养的细胞光谱中还检测到HA的荧光峰 ,提示HA在癌细胞中滞留。发展了利用显微光谱研究细胞在药物作用下的变化这一技术。Microspectra of mouse lymph cancer cells which one is cultured with HA and the other is cultured without HA have obtained in the range of 20 000-12 000 cm(-1). The result shows that two microspectra are guile different. This point out the cancer cell has changed because of HA. In the microspectrum of cancer cell cultured with HA,we have got the fluorescent peak of HA. This prompts HA has detained in the cancer cell. A new method which studies cells after being affected by drugs using microspectrum has also being developed.

关 键 词：竹红菌甲素 细胞显微光谱 癌细胞 光动力疗法 滞留效应 癌症 光效药物 

分 类 号：R979.1[医药卫生—药品] R730.5[医药卫生—药学]