镍离子注入修饰电极上米托蒽醌与DNA相互作用的电化学研究  

Electrochemical Studies of Mitoxantrone Interaction with DNA at a Ni/GC Ion Implantation Modified Electrode

作　　者：胡劲波[1] 尚军[1] 李启隆[1] 

机构地区：[1]北京师范大学化学系,北京100875

出　　处：《高等学校化学学报》2001年第5期749-753,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :2 9875 0 0 3 );教育部博士学科点基金! (批准号 :980 0 2 70 9)资助

摘　　要：研究了在镍离子注入修饰电极和 0 .0 5mol/L Tris-0 .5mol/L Na Cl缓冲溶液 (p H=7.1 )中 ,米托蒽醌(MX)与 DNA作用的电化学 .在 3 7℃恒温 1 .5h条件下 ,MX与 DNA形成一种非电活性的结合物 ,使 MX峰电流降低 ,其结合比 n(MX)∶n(DNA) =2∶ 1 ,结合常数为 1 .61× 1 0 12 ,电子转移系数为 0 .4 1 ,电极反应速率常数 0 .3 3 s-1.加入 DNA后 ,MX峰电流降低 ,据此 ,可以测定 DNA.The electrochemical behavior of mitoxantrone interaction with DNA at a Ni/GC modified electrode was studied by linear sweep and cyclic voltammetry. In 0.05 mol/L Tris 0.5mol/L NaCl solution (pH=7.1), the reaction of DNA with MX formed an electrochemically nonactive complex, resulting in a decrease in the MX equilibrium concentration and its reduction current. The composition of the complex was n (MX)∶ n (DNA)=2∶1. The combining constant was 1.61×10 12 . The electrode reaction rate constant k 0 and the electron transfer coefficient α were 0.33 s -1 and 0.41, respectively. The decrease in peak current was proportional to DNA concentration and can be used to determine DNA concentration.

关 键 词：米托蒽醌 DNA 电化学行为 相互作用 抗癌药 镍离注入修饰电极 

分 类 号：R979.1[医药卫生—药品] R96[医药卫生—药学]