带活性末端的热敏高分子的制备及其在荧光免疫分析中的应用  

Preparation of Temperature-sensitive Polymer with Reactive End Carboxylic Group and Its Application in the Fluoroimmunoassay

作　　者：杨黄浩[1] 朱庆枝[1] 李东辉[2] 丁马太[1] 许金钩[1] 

机构地区：[1]厦门大学化学化工学院现代分析教育部重点实验室 [2]厦门大学抗癌研究中心,厦门大学化学系厦门361005

出　　处：《高等学校化学学报》2001年第5期754-758,共5页Chemical Journal of Chinese Universities

基　　金：教育部博士点基金! (批准号 :970 3 840 7;福建省自然科学基金! (批准号 :B9810 0 0 4)资助

摘　　要：合成了带有活性末端的寡聚 N-异丙基丙烯酰胺 (ONIPAAm) ,并考察了其温度敏感性质 .以 ONI-PAAm作为免疫分析的载体与鼠 Ig G偶联 ,以四磺基铁酞菁为标记物 ,以羊抗鼠 Ig G抗体为分析模型 ,建立了竞争型热敏相分离荧光免疫分析新系统 .羊抗鼠 Ig G抗体在 0～ 1 50 0 ng/m L范围内与体系相对荧光强度呈良好的线性关系 ,检测限为 2 ng/m L.A temperature sensitive polymer containing a terminal carboxyl group was prepared by copolymerization of N isopropylacrylamide with mercaptoacetic acid. This polymer was conjugated to the IgG with EDCI and used to develop a novel polymer mimetic enzyme competitive immunoassay method for determination of anti IgG with iron tetrasulfonatophthalocyanine as a labelling reagent to catalyze the reaction of p hydroxyphenyl acetic acid (HPA) and hydrogen peroxide in alkaline medium. The calibration graph for anti IgG was linear over the range of 0\_1 500 ng/mL with a detection limit of 2.0 ng/mL.

关 键 词：寡聚N-异丙基丙烯酰胺 制备 羊抗鼠IgG抗体 热敏高分子 热敏相分离 模拟酶 荧光免疫分析 

分 类 号：O633.22[理学—高分子化学] R446.62[理学—化学]