胶束增敏催化荧光法测定血红蛋白  

Micelle Enhanced Catalytic-Fluorimetric Determination of Hemoglobin

作　　者：王全林[1] 刘志洪[2] 原弘[2] 蔡汝秀[2] 

机构地区：[1]固原师范高等专科学校化学系,756000 [2]武汉大学化学与环境科学学院,武汉430072

出　　处：《分析化学》2001年第4期421-424,共4页Chinese Journal of Analytical Chemistry

摘　　要：基于血红蛋白（Ｈｂ）的过氧化物酶活性，催化过氧化氢氧化对甲基酚的反应体系，发现表面活性剂ＳＤＳ对该体系反应速率有明显的增强效应；从而建立了新的高灵敏测定Ｈｂ的催化荧光分析法。在１．５×１０－３ｍｏｌ／Ｌ　ＳＤＳ存在下，测定Ｈｂ的线性范围１×１０－９～８×１０－８ｍｏｌ／Ｌ，检出限为３．４×１０－１０ｍｏｌ／Ｌ，用于尿中Ｈｂ的测定，结果满意。A highly sensitive catalytic-fluorimetric method based on Hemoglobin (Hb) enzymatic activityto catalyze the oxidation of p-Cresol with hydrogen peroxide for the determination of hemoglobin in the presence of sodium dedecyl sulphonate (SDS) has been developed, indicated that the initial rate of this reaction was accelerated with the addition of SDS. The linear relationship b etween the initial reaction rate and the hemoglobin concentration was in the range of 1×10-9～8×10-8mol/L. The detection limit was 3.4×10-10mol/L. The method was used to determine hemoglobin in the urine samples with satisfactory result.

关 键 词：胶束增敏 过氧化氢 血红蛋白 测定 尿 临床分析 对甲基酚 催化荧光分析法 

分 类 号：O657.3[理学—分析化学]