反相高效液相色谱法和紫外分光光度法测定氟罗沙星滴眼液的含量  

RP-HPLC and UV determination of fleroxacin eye drops

作　　者：任志强[1] 李伟[1] 姜虹[1] 李宪为[1] 肖科武[1] 郭利民[1] 

机构地区：[1]益阳市中心医院,湖南益阳413000

出　　处：《中国医院药学杂志》2001年第5期279-280,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立氟罗沙星滴眼液的含量测定方法。方法 :UV法 :在 2 86波长处测定 ;HPLC法 :以甲硝唑为内标 ,0 .0 5mol·L-1柠檬酸 乙腈 ( 82∶18)为流动相 ,在 2 86nm波长处测定。结果 :相关系数γ分别为 0 .9999,0 .9997;平均回收率分别为10 0 .2 6% ,10 0 .12 % ;RSD分别为 1.3 % ,1.0 2 %。结论 :该二法均可作为氟罗沙星的含量测定。OBJECTIVE To establish a method forthe fleroxacin eye drops.METHODS UV:The determination wave length was 286 nm.HPLC:Using metronidazole as interal standard. The mobile phase was 0.05 mol·L -1 citratis acid acetonitrile (82∶18).RESULTS The correlation coefficient were 0.9999 , 0.9997 ;the average recoveries were 100.28% , 100.12% ;RSD were 1.03% , 1.02% .CONCLUSIONS These two methods were suitable for the determination of thefleroxacin eye drops.

关 键 词：氟罗沙星滴眼液 高效液相色谱法 紫外分光光度法 含量 测定 

分 类 号：R988.1[医药卫生—药品] R927.2[医药卫生—药学]