移植肾慢性排斥纤维化中巨噬细胞和NO作用机制的探讨  

Role of the macrophages and NO in fibrosis formation in transplanted kidneys with chronic rejection

作　　者：刘会敏[1] 叶廷军[1] 陈泳莲[1] 

机构地区：[1]第二军医大学长征医院病理科,上海200003

出　　处：《第二军医大学学报》2001年第4期370-372,共3页Academic Journal of Second Military Medical University

摘　　要：目的 :探讨移植肾慢性排斥中纤维化过程的形成机制。 方法 :采用免疫组化技术及图像分析技术 ,观察移植肾慢性排斥形成过程中巨噬细胞的形态学特征及 NO含量的变化。 结果 :移植肾慢性排斥早、中期 ,损害的肾小球、肾小管处巨噬细胞增多、聚集 ,NO表达率增加 , 、 型胶原的表达增强 ;后期 ,已硬化的肾小球、肾小管处的巨噬细胞无 NO表达 , 、 型胶原的表达减弱。 CD6 8阳性细胞计数 ,NO以及 、 型胶原的含量在移植性肾小球病型的肾小球以及肾间质硬化型的肾小管中最高。 结论 :移植肾慢性排斥形成过程中巨噬细胞增多且 NO表达增加 ,可能与肾纤维化形成有关。Objective:To confirm the effects of the macrophages and NO in the fibrosis formation of transplanted kidneys with chronic rejection. Methods:The quantitive immunohistochemistry was employed to observe the changes of relative quantitiy of macrophages, NO, collagen Ⅲ andⅣ, and to reveal the association between the increases of macrophages, NO, histopathologic damages, deposition of collagen Ⅲ and Ⅳ, finally, to infer the roles of the macrophages and NO in the fibrosis formation of transplanted kidneys with chronic rejection. Results: The macrophages accumulated and increased in the glomeruli, the tubules and the intersiticia tissue of kidneys, which was companied by the increased expression of collagen Ⅲ and Ⅳ during the early and middle stages. However, the macrophages and NO could not be detected, which was companied by the decreased deposition of collagen Ⅲ and Ⅳduring the advanced stage. There were significant differences between the 3 types(glomerulus disease type,blocking vessel type and intersticial sclerostic type) ( P <0.05 or P <0.01). Conclusion:The increase of macrophages and the NO may closely interrelated with the fibrosis formation in transplanted kidneys with chronic rejection. [

关 键 词：肾移植 移植物排斥 巨噬细胞 一氧化氮 纤维化 

分 类 号：R699.2[医药卫生—泌尿科学] R392.4[医药卫生—外科学]