高效液相色谱法同时测定美容霜中3种有效成分  

Simultaneous determination of three effective constituents in Meirong Shuang by HPLC

作　　者：黄品芳[1] 王长连[1] 刘亦伟[1] 

机构地区：[1]福建医科大学附属第一医院,福建福州350005

出　　处：《中国医院药学杂志》2001年第4期209-210,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :测定美容霜中替硝唑、西咪替丁、氯霉素三者的含量。方法 :高效液相色谱法 ,以苯巴比妥为内标 ,Nova pakC18柱 ,甲醇 水 冰乙酸 二正丁胺为流动相 ;检测波长 2 0 5nm。结果 :一次进样可完成 3种化学性质不同组分的含量测定。替硝唑 1.5 75～ 2 5 .0 μg·ml-1,西咪替丁 3.15～ 5 0 .0 μg·ml-1,氯霉素 0 .945～ 15 .0 μg·ml-1浓度范围内 ,每个组分与内标峰面积的比与其浓度呈良好的线性关系 ;平均回收率分别为 10 0 .9%(RSD 1.2 %) ;98.0 %(RSD 0 .7%) ,91.0 %(RSD 0 .8%)。结论 :方法简便、快速、准确 。OBJECTIVE To separate and determine three constituents including tinidazole, cimetidine and chloramphenicol in Meirong Shuang . METHODS An HPLC was used. Phenpbabital was used as internal standard.Chromatographic conditions included Nova pak C 18 column and the mobile phase consisting of methanol water glacial acetic acid dibutylamine(30∶70∶ 0.1 ∶ 0.0011 );detector,UV205 nm.RESULTS The calibration curves were linear in the range of 1.575 ～ 25.0 μg·ml -1 for tinidazole; 3.15 ～ 50.0 μg·ml -1 for cimetidin and 0.945 ～ 15 μg·ml -1 for chloramphencol.The average recoveries were 100.95% ( RSD = 1.2% ); 98.0% (RSD = 0.7% ); 10.0% ( RSD = 0.8% ) respectively. CONCLUSIONS This method was found to be simple,and accurate for simultaneous determination of the three constituents in Meirong Shuang .

关 键 词：替硝唑 西咪替丁 氯霉素 高效液相色谱法 美容霜 

分 类 号：R986[医药卫生—药品] R927.2[医药卫生—药学]