人尿集落刺激因子的纯化及性质  

Studies on purification and some properties of colony stimulating fac-tor from normal human urine

作　　者：厉朝龙[1] 王志勤[1] 邵传森[1] 蔡菊芬 

机构地区：[1]浙江医科大学,浙江省肿瘤医院

出　　处：《药物生物技术》1995年第1期10-15,共6页Pharmaceutical Biotechnology

基　　金：浙江省自然科学基金

摘　　要：本文报道了从人尿中提取纯化获得一种ＣＳＦ，它能促进小鼠骨髓有核细胞增殖分化，形成粒巨噬系细胞集落。提取纯化方法包括超滤浓缩、硫酸铵盐析、ＤＥＡＥ－纤维素柱层析、凝胶过滤及聚丙烯酰胺凝胶电泳等，所得ＣＳＦ纯化约１５００倍。经ＳＤＳ－ＰＡＧＥ测定分子量为６０，０００道尔顿，用等电点聚焦技术测得ｐＩ为４．３５，比活性为２．４７×１０４Ｕ／ｍｇ蛋白质。人尿中存在集落抑制因子（ＣＩＦ），该因子可经ＤＥＡＥ纤维素柱层析与ＣＳＦ有效分离。经集落形成抑制试验证明，ＣＩＦ对ＣＳＦ刺激粒巨噬系祖细胞的增殖分化具有明显的抑制作用。CSF that stimulated mouse bone marrow cells to proliferate in vitro and form colonies of granulocyteand /or macrophage was purified about 1500-fold from normal human urine. Purification proceduresincluded concentration with ultrafiltration, ammonium sulfate precipitation, chromatographic separa-tion on DEAE-cellulose, and gel filtration. The molecular weight of the purified factor was estimatedto be about 60000 dalton by SDSPAGE, the p1 was estimated to be pH 4. 35 by isoelectric focusing,and the specific activity was found to be 2. 47× 104U/mg protein using mouse bone marrow cells,re-spectively. A urinary colony inhibiting factor (CIF)was separated from the CSF on the DEAE-cellu-lose column. This CIF suppressed the formation of granulocyte/ macrophage colonies of mouse bonemarrow cells when employed in conjunction with the purified urinary CSF.

关 键 词：集落刺激因子 分离 纯化 集落抑制因子 人尿 

分 类 号：Q592.2[生物学—生物化学] Q516