严重多发伤后循环内皮细胞体外培养及其影响因素  

In vitro culture of circulating endothelial cells and its influential factors after severe multiple trauma

作　　者：周健[1] 林水金[1] 杨映波 冯素珍[1] 简华刚[1] 

机构地区：[1]第三军医大学附属大坪医院战创伤中心

出　　处：《第三军医大学学报》1998年第1期16-18,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：探讨严重多发伤后循环内皮细胞（ＣＥＣ）的体外培养及其影响因素。方法：对１７例严重多发伤（ＩＳＳ≥２０）病人外周血中ＣＥＣ进行了分离、鉴定和培养。结果：培养总成功率为４３．３％，而ＩＳＳ≥３０者为６２．５％。伤情愈重，培养成功率愈高。影响ＣＥＣ体外培养成败的因素是，损伤严重程度、采血时间、分离和培养技术。结论：本研究通过完善ＣＥＣ分离、培养技术，克服不利影响因素，提高了培养成功率，为进一步研究ＣＥＣ表面成份奠定了基础，并提供了新的途径。Objective: To explore in vitro culture of circulating endothelial cells (CECs) and its influential factors after severe multiple trauma. Methods: After being isolated from the peripheral blood in 17 patients with severe multiple trauma (ISS≥20), the CECs were differentiated and then cultured in vitro. Meanwhile, the influential factors on the culture were observed and analyzed. Results: The total successful culturing rate was 62.5%. The severer the trauma was, the higher the successful culturing rate was. The influential factors on the culture were severity of trauma, time for blood sampling and techniques of isolation and culture. Conclusion: In this study, the successful rate of in vitro culture of CECs was increased through improving the techniques of isolation and culture and overcoming the unfavorable influential factors. This provides a new method for further study on the surface components of CECs.

关 键 词：循环内皮细胞 体外培养 影响因素 CEC 多发伤 

分 类 号：R641[医药卫生—外科学]