枸杞子多糖及合并应用厌氧短棒杆菌菌苗对小鼠腹腔巨噬细胞抑制肿瘤增殖活性的影响(英文)  

Effects of Lycium barbarum polysaccharides and their combination with Corynebacterium parvum on the tumoristatic activity of peritoneal macrophages in mice

作　　者：张永祥[1] 邢善田[1] 周金黄[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京1000850

出　　处：《中国药理学与毒理学杂志》1989年第3期169-174,共6页Chinese Journal of Pharmacology and Toxicology

摘　　要：本研究观察了中药枸杞子(Lyciumbarbarum L.)提取物枸杞多糖(LBP)对小鼠腹腔巨噬细胞抑制肿瘤增殖活性的影响。结果表明,给正常小鼠ip LBP 40 mg/kg×7d能增强经Con A处理的巨噬细胞抑制肿瘤靶细胞增殖的活性;LBP 5.10.20,40mg/kg×7 d并与小剂量(250μg/只)厌氧短棒杆菌菌苗(Corynebacterium parvum,CP)合并应用,具有明显的协同效应。在LBP浓度为20 mg/kg时,效应最为显著,对靶细胞P815及P388增殖的抑制率分别为85.5％和63.6％;CP对照组则为28.1％及24.0％。表明合并应用LBP及CP可减少二者的用量,增强效应,减低CP的毒副作用。据本室先前的研究发现,LBP为主要作用于T细胞的免疫增强剂,能增强CTL,NK细胞的功能;CP则为巨噬细胞刺激剂。本研究结果提示,合并应用作用于免疫应答不同环节的免疫增强剂可获得协同效应,这将为临床肿瘤的免疫疗法中免疫增强剂的联合应用提供参考。此外,本实验还发现,来自经肿瘤细胞免疫小鼠的腹腔巨噬细胞,表现出较强的特异性抑制肿瘤增殖活性,LBP则能进一步加强其作用。以上结果提示,LBP无论对巨噬细胞在非特异性抗肿瘤或特异性抗肿瘤过程中,均具有激活作用。Lycium barbarian polysaccharidcs(LBP)extracted from Lycium barbarum L.is a tonic with potentiating effect on cellular immunological functions.In our experiments,we found that LBP could increase tumoristatic activity of peritoneal macrophagcs(Mφ)treated with Con A in vitro against P815 cells.LBP(5,10,20,40 mg/kg)combined with a low dose of Corynebacterium parvum(CP)expressed a synergistic effect on the cytostatic activity of Mφ against both P815 and P388 cells.The maximal effect was observed when the dosage of LBP was 20 mg/kg.The cytostasis(%)of Mφ against P815 and P388 cells were 85.5 and 63.6,while 28.1 and 24.0 in CP controls respectively.The results indicated that LBP could activate peritoneal Mφ of mice to a certain extent and LBP combined with a small dose of CP showed a synergistic effect which was helpful to augment tumoristatic activity of Mφ and to decrease the toxic action of CP,thereby suggesting a new way in immunothcrapy of cancer.In addition,Mφ obtained from C57BL/6 mice immunized with P815 cells(immune-Mφ)showed obviously cytostatic activity to P815 cells and such activity of immune-Mφ was markedly intensified by LBP.

关 键 词：枸杞子多糖 厌氧短棒杆菌菌苗 腹腔巨噬细胞 抑制肿瘤增殖活性 协同效应 

分 类 号：R285.5[医药卫生—中药学]