放烧复合伤骨髓基质细胞对骨髓单个核细胞的粘附能力及GM┐CFU形成的影响  

Effects of stromal cells on adherent capacity of mononuclear cells and GM CFU growth in the bone marrow after combined radiation burn injury in mice

作　　者：陈幸华[1] 罗成基[1] 郭朝华[1] 王苹 王庆余[1] 刘林[1] 彭贤贵[1] 曹正怀 邓学才[2] 

机构地区：[1]第三军医大学预防医学系防原医学教研室 [2]第三军医大学基础医学部

出　　处：《第三军医大学学报》1998年第3期217-219,共3页Journal of Third Military Medical University

基　　金：全军"八五"指令性攻关项目

摘　　要：目的：观察放烧复合伤小鼠骨髓基质细胞贴壁层的粘附能力及对ＧＭ－ＣＦＵ（粒单系造血组细胞集落）形成的影响。方法：采用细胞粘附实验和细胞粘附阻断实验方法。检测５Ｇｙγ线全身照射、１５％体表面积Ⅲ度烧伤和放烧复合伤小鼠骨髓基质细胞粘附的功能。结果：单烧组伤后第３～７天显著高于正常组（Ｐ＜０．０５～０．０１）；单放组伤后第３～７天显著低于正常组（Ｐ＜０．０５～０．０１）；复合伤组伤后第３天显著低于正常组（Ｐ＜０．０１）；正常组骨髓基质细胞贴壁层分别加入ＶＣＡＭ－１、Ｆｎ、Ｌｎ、ＣｏｌⅣ等单抗或ＶＣＡＭ－１单抗分别联合Ｆｎ、Ｌｎ和ＣｏｌⅣ等单抗处理后，ＧＭ－ＣＦＵ数量均有不同程度的降低。Objective: To investigate the adherent capacity of mononuclear cells and GM CFU growth on stromal cell adherence layer in the bone marrow after combined radiation burn injury using in vitro culture. Methods: The bone marrow stromal cells extracted from mice inflicted with 5.0 Gy γ ray radiation injury, 15% TBSA full thickness skin burns and combined radiation burn injury respectively were cultured and detected with cell adhesion assay and cell adhesion blocking technique. Results: The adherent capacity of the mononuclear cells from the mice with simple burns was higher than that from the normal controls and significantly increased at the period from the 3rd to 7th d after injury as compared with those from the normal controls, mice with simple radiation injury and mice with combined radiation burn injury (P<0.05～0.01). Both of the last two were significantly decrease as compared with that from the normal controls (P<0.05～0.01). After the stromal adhesion layer in the bone marrow from the normal controls was added with such monoclonal antibodies (McAbs) as VCAM 1, Fn, Ln and Col IV respectively or VCAM 1 in combination with Fn, Ln and Col IV respectively, the number of CFU GM was decreased in various degrees. Conclusion: The decrease in adherent capacity of bone marrow hematopoietic indusive microenvironment might be one of the important reasons for hematopoietic disorder after combined radiation burn injury.

关 键 词：放烧复合伤 骨髓 基质细胞 细胞粘附 小鼠 GM-CFU 

分 类 号：R818.74[医药卫生—放射医学] R644[医药卫生—临床医学]