虎杖甙对正常大鼠血管平滑肌细胞内游离钙浓度的影响  

The effects of polydatin on free calcium concentration of aorta smooth muscle cells in rats

作　　者：金春华[1] 赵克森[1] 刘杰[1] 黄辉[1] 朱佐江[1] 黄绪亮[1] 

机构地区：[1]第一军医大学病理生理教研室

出　　处：《中国病理生理杂志》1998年第2期195-198,共4页Chinese Journal of Pathophysiology

摘　　要：目的：观察虎杖甙（ＰＤ）对培养的大鼠血管平滑肌细胞（ＶＳＭＣ）内钙离子浓度的变化机理。方法：用Ｆｌｕｏ－３－ＡＭ标记培养的ＶＳＭＣ，在粘附式细胞仪上测定细胞内游离钙的变化。结果：实验结果表明，ＰＤ（００２～２０ｍｍｏｌ／Ｌ）可使大鼠ＶＳＭＣ内游离钙浓度升高，波形变宽，其引起的钙波形态与去甲肾上腺素（ＮＥ）及氯化钾所引起的高尖钙波不同，ＰＤ使ＶＳＭＣ产生一种剂量依赖性的、持续缓慢升高的钙波。ＰＤ引起的细胞内游离钙升高可被ＥＧＴＡ（２ｍｍｏｌ／Ｌ）及维拉帕米（５０μｍｏｌ／Ｌ）明显抑制。结论：以上结果提示ＰＤ既促进ＶＳＭＣ外钙离子进入细胞内，还能诱导细胞内钙离子释放；ＰＤ可能会提高正常ＶＳＭＣ的收缩性，增加正常血管的张力。AIM:The present study was to investigate the mechanism of polydatin effects on vessels. METHODS:The changes of the cytosolic free calcium concentration of cultured vascular smooth muscle cells (VSMC)were determined kinetically from rat aorta.RESULTS:It was found that polydatin caused a persistent and dose-dependent increase of intracellular calcium concentration[Ca 2+ ]i,which were inhibited partly by pretreatment with calcium chelator EGTA(2 mmol/L) and calcium channel blocker verapamil(50 μmol/L)respectively 10 min prior to polydatin administration. CONCLUSION:The results suggested that polydatin-induced [Ca 2+ ]i alternation are not only due to extracellular Ca 2+ inflow but also Ca 2+ release from intracellular calcium store.

关 键 词：虎杖甙 大鼠 血管平滑肌细胞 游离钙 

分 类 号：R543[医药卫生—心血管疾病] R285.5[医药卫生—内科学]