构建人MHC-Ⅱ类基因反义RNA的逆转录病毒重组体  

Construction of retroviral vector for expressive HLA-DR β gene antisense RNA

作　　者：邓宇斌[1] 李树浓[1] 黄绍良[1] 黄仁魏[1] 

机构地区：[1]中山医科大学病理生理学教研室移植免疫研究室

出　　处：《中国病理生理杂志》1998年第3期235-238,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金;中国博士后基金

摘　　要：目的：探讨运用反义ＲＮＡ技术降低脐血细胞ＭＨＣ－Ⅱ类基因的表达是否能有效减少ＧＶＨＲ发生的危险程度，为临床上进行脐血移植降低脐血干细胞的移植物抗宿主反应提供了理论依据和实验基础。方法：应用分子克隆技术构建了含人ＨＬＡ－ＤＲβ基因的逆转录病毒表达载体，经过狭缝杂交、电泳分析、酶切图谱分析鉴定。结果：获得了ＨＬＡ－ＤＲβ基因反义ＲＮＡ重组体。结论：构建人ＭＨＣ－Ⅱ类基因反义ＲＮＡ的逆转录病毒重组体。AIM:This study is aimed at looking for the possibility of reducing graft versu host reaction risk through lowering the exprssion of MHC-Ⅱ gene (HLA-DR β)METHODS:Human HLA-DR β gene cDNA was isolated by using restriction enzyme BamHI from plasmid pCDV and was inserted into BamHI stie of retroviral vector pZIP-neo to construct a recombinant plasmid pZIP-neo/HLA-DR β. It was identified by agarose gel electrophoresis, restriction enzyme Hind Ⅲ analysis and slot hybridization. RESULTS: pZIP-neo /HLA-DR β contained the inserted HLA-DR cDNA fragment. CONCLUSION: The retroviral vector for expressive HLA-DR β gene antisense RNA was constructed. This study provides a foundation for using recombinant retrovirus mediated antisense HLA-DR β gene transfer and gene therapy.

关 键 词：核糖核酸 逆转录病毒科 造血干细胞移植 GVHR MHC-Ⅱ类基因 反义RNA 基因重组 

分 类 号：R457[医药卫生—治疗学]