兔盲肠结扎穿孔早期一氧化氮抗肺动脉压增高和抗氧化作用  

Anti-pulmonary hypertension and antioxidant action of nitric oxide during early stage of cecal ligation and puncture in rabbits

作　　者：万梅[1] 凌亦凌[1] 黄善生[1] 张君岚[1] 郝荣利 

机构地区：[1]河北医科大学病理生理教研室

出　　处：《中国病理生理杂志》1998年第3期250-253,共4页Chinese Journal of Pathophysiology

摘　　要：目的和方法：采用家兔盲肠结扎穿孔（ＣＬＰ）模型观察了ＣＬＰ前后以及一氧化氮（ＮＯ）合成抑制剂左旋硝基精氨酸（Ｌ－ＮＮＡ）对平均动脉血压（ＭＡＰ），肺动脉压（ＰＡＰ），入、出肺血ＮＯ、丙二醛（ＭＤＡ）含量和超氧化物歧化酶（ＳＯＤ）活性的改变。结果：兔ＣＬＰ后１～５ｈＭＡＰ明显下降，而在２、２５ｈ出现ＰＡＰ明显升高。ＣＬＰ前出肺血ＮＯ含量显著低于入肺血，ＣＬＰ后２５ｈ入肺血ＮＯ含量低于ＣＬＰ前，而出肺血ＮＯ含量与ＣＬＰ前相比无明显差异，且入、出肺血ＮＯ含量无明显差异。ＣＬＰ后２５ｈ入肺血ＭＤＡ含量比ＣＬＰ前有明显增加，出肺血无显著改变；而出肺血ＳＯＤ活性比ＣＬＰ前有明显增高，入肺血无显著改变。注入Ｌ－ＮＮＡ后入、出肺血ＮＯ含量明显降低，各时点ＰＡＰ都明显增高，５ｈ生存率降低，同时入、出肺血ＭＤＡ含量明显升高，ＳＯＤ活性明显下降。结论：肺内ＮＯ含量改变可能与氧自由基有关。AIM and METHOD:Mean artery pressure(MAP),pulmonary artery pressure(PAP), content of NO,malondialdehyde(MDA) and superoxide dismutase(SOD) of in-flowing and out-going pulmonary blood(IPB,OPB) were observed respectively before and after rabbit cecal legation and punction (CLP) as well as after nitric oxide (NO) inhibition (L-NNA injection).RESULTS:MAP decreased 1～5h after CLP,while PAP increased significantly 2～2 5h after CLP.NO content in OPB was much lower than IPB before CLP,but the change was not significant 2 5h after CLP and the NO content in IPB was lowered compared with that before CLP.MDA content in IPB was increased but that in OPB had no change after CLP.In contrast,SOD content in OPB increased but that in IPB had no change after L-NNA injection NO content in IPB and OPB was lower,PAP was increased ,5h'Survival rate was lower,MDA was higher,SOD was lower sinificantly. CONCLUSION:The NO content may be associated with oxygen free radical.NO has the effects against pulmonary hypertension and antioxidant action in lung during early stage of CLP.

关 键 词：一氧化氮 高血压 游离基类 腹膜炎 败血症休克 抗氧化作用 肺动脉高压 CLP 盲肠结扎穿孔 

分 类 号：R656.41[医药卫生—外科学] R631.3[医药卫生—临床医学]